Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GFP antibody

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
Yes - Triton X-100 (0.3%) in blocking buffer
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C

Other product details

Dilution
1/200
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: In blocking buffer

Secondary antibody

Name
Non-Abcam antibody was used: Donkey anti-Goat
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Dilution
1/200

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Submitted Dec 19 2012

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