Western blot

Good
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Non-reduced Denaturing (10)
Sample
Human Cell lysate - whole cell (HeLa cells)
Specification
HeLa cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS-Tween
Dilution
1/2000

Secondary antibody

Name
Non-Abcam antibody was used: Dako Rabbit Anti-Goat
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL
Bands
Specific: 25 kDa
Exposure
1 minute(s) and 0 second(s)

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Submitted May 28 2013

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