Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GFP antibody

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (esophagus)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM citric acid, pH6.0
Specification
esophagus
Blocking step
Non-Abcam blocking agent as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C
Fixative
Formaldehyde

Other product details

Incubation time
17 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS+0.1%BSA+0.2%TritonX-100
Dilution
1/5000

Secondary antibody

Dilution
1/200
Name
Non-Abcam antibody was used: Biotinylated anti-goat antibody produced in rabbit
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Biotin

Additional data

Additional Notes
The sample is esophagus from a mouse expressing GFP under K15 promoter, which labels a fraction of cells in the basal layer of esophageal epithelium. Even at a 1:5000 dilution, there is still a lot of background with keratinized layer

Tatiana Karakasheva

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Submitted Feb 22 2016

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