Key features and details
- Chicken polyclonal to GFP
- Suitable for: ELISA, ICC, WB
- Reacts with: Species independent
- Isotype: IgY
Product nameAnti-GFP antibody
See all GFP primary antibodies
DescriptionChicken polyclonal to GFP
Tested applicationsSuitable for: ELISA, ICC, WBmore details
Species reactivityReacts with: Species independent
Recombinant full length protein corresponding to Aequorea victoria GFP.
Database link: P42212
- Green Fluorescence Protein
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab92456 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC: 1/200 - 1/1000.
WB: 1/500 - 1/2000. Predicted molecular weight: 27 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceFunction: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+ -activated photoprotein aequorin.
Subunit structure: Monomer.
Tissue specificity: Photocytes.
Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.
Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.
Sequence similarities: Belongs to the GFP family.
Biophysicochemical properties: Absorption: Abs(max)=395 nm
Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm.
- GFP antibody
- Green fluorescent protein antibody
ab92456 has been referenced in 2 publications.
- Trébuchet G et al. The Repo Homeodomain Transcription Factor Suppresses Hematopoiesis in Drosophila and Preserves the Glial Fate. J Neurosci 39:238-255 (2019). PubMed: 30504274
- Lee JK et al. Abelson tyrosine-protein kinase 2 regulates myoblast proliferation and controls muscle fiber length. Elife 6:N/A (2017). PubMed: 29231808