Product nameAnti-GFP antibody (Biotin)
See all GFP primary antibodies
DescriptionRabbit polyclonal to GFP (Biotin)
SpecificityThis product is affinity purified antibody (ab6556) covalently linked to biotin. It is reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, RFP and EGFP.
Tested applicationsSuitable for: IHC-FoFr, WBmore details
Recombinant full length protein corresponding to GFP.
Database link: P42212
This antibody is a biotin conjugated version of ab6556.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 8.00
Constituents: 0.7946% Sodium chloride, 0.3025% Tris
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody is an affinity purified rabbit anti-GFP antibody purified on an affinity chromatography column made with highly purified recombinant GFP.
Our Abpromise guarantee covers the use of ab69313 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 20824308|
|WB||1/25000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa). An unconjugated version of this antibody is available as ab6556. FITC conjugated version: ab66180. HRP conjugated version: ab69312. Sepharose version: ab69314. Magnetic beads version: ab69315.|
RelevanceFunction: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+ -activated photoprotein aequorin.
Subunit structure: Monomer.
Tissue specificity: Photocytes.
Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.
Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.
Sequence similarities: Belongs to the GFP family.
Biophysicochemical properties: Absorption: Abs(max)=395 nm
Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm.
- GFP antibody
- Green fluorescent protein antibody
Western blots results from a 12.5% SDS-PAGE gels loaded with highly purified GFP (antigen) or transiently transfected COS-7 cell lysates expressing truncated Yes-GFP (1) or empty vector as shown. Membranes were probed with rabbit anti-GFP-biotin conjugate at a ratio of 1/25,000 (100 ng/2.5 ml), followed by NeutrAvidin™-HRP conjugate at a ratio of 1/50,000 (100 ng/5 ml) and ECL. Developed membranes were exposed to Kodak X-OMAT™ LS film for 15 seconds. Shown on the right is the PVDF membrane stained with Coomassie blue.
Lanes 1-3: PVDF membrane probed with rabbit anti-GFP-biotin (1/25,000) allows detection of 25 ng GFP antigen (M.W.~27kDa).
Lanes 4-7: : PVDF membrane probed with rabbit anti-GFP-biotin (1/25,000) allows detection of Yes-GFP protein expressed using 1 and 10 ug of appropriately transiently transfected COS-7 cell lysates.
Note: the bands present at an apparent molecular weight of ~75 kDa and 150 kDa are endogenously biotinylated proteins
This product has been referenced in:
- Sztainberg Y et al. An Anxiolytic Role for CRF Receptor Type 1 in the Globus Pallidus. J Neurosci 31:17416-24 (2011). Read more (PubMed: 22131403) »
- Zhang H et al. Multifunctional peptide-PEG intercalating conjugates: programmatic of gene delivery to the blood-brain barrier. Pharm Res 27:2528-43 (2010). IHC-FoFr ; Mouse . Read more (PubMed: 20824308) »