• Product name
    Anti-GFP antibody (Biotin)
    See all GFP primary antibodies
  • Description
    Rabbit polyclonal to GFP (Biotin)
  • Host species
  • Conjugation
  • Specificity
    This product is affinity purified antibody (ab6556) covalently linked to biotin. It is reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, RFP and EGFP.
  • Tested applications
    Suitable for: IHC-FoFr, WBmore details
  • Immunogen

    Recombinant full length protein corresponding to GFP.
    Database link: P42212

  • General notes

    This antibody is a biotin conjugated version of ab6556.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 8.00
    Constituents: 0.7946% Sodium chloride, 0.3025% Tris
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody is an affinity purified rabbit anti-GFP antibody purified on an affinity chromatography column made with highly purified recombinant GFP.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab69313 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 20824308
WB 1/25000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa). An unconjugated version of this antibody is available as ab6556. FITC conjugated version: ab66180. HRP conjugated version: ab69312. Sepharose version: ab69314. Magnetic beads version: ab69315.


  • Relevance
    Function: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+ -activated photoprotein aequorin.

    Subunit structure: Monomer.

    Tissue specificity: Photocytes.

    Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

    Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.

    Sequence similarities: Belongs to the GFP family.

    Biophysicochemical properties: Absorption: Abs(max)=395 nm
    Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm.
  • Alternative names
    • GFP antibody
    • Green fluorescent protein antibody


  • Western blots results from a 12.5% SDS-PAGE gels loaded with highly purified GFP (antigen) or transiently transfected COS-7 cell lysates expressing truncated Yes-GFP (1) or empty vector as shown. Membranes were probed with rabbit anti-GFP-biotin conjugate at a ratio of 1/25,000 (100 ng/2.5 ml), followed by NeutrAvidin™-HRP conjugate at a ratio of 1/50,000 (100 ng/5 ml) and ECL. Developed membranes were exposed to Kodak X-OMAT™ LS film for 15 seconds. Shown on the right is the PVDF membrane stained with Coomassie blue.

    Lanes 1-3: PVDF membrane probed with rabbit anti-GFP-biotin (1/25,000) allows detection of 25 ng GFP antigen (M.W.~27kDa).

    Lanes 4-7: : PVDF membrane probed with rabbit anti-GFP-biotin (1/25,000) allows detection of Yes-GFP protein expressed using 1 and 10 ug of appropriately transiently transfected COS-7 cell lysates.

    Note: the bands present at an apparent molecular weight of ~75 kDa and 150 kDa are endogenously biotinylated proteins


This product has been referenced in:
  • Sztainberg Y  et al. An Anxiolytic Role for CRF Receptor Type 1 in the Globus Pallidus. J Neurosci 31:17416-24 (2011). Read more (PubMed: 22131403) »
  • Zhang H  et al. Multifunctional peptide-PEG intercalating conjugates: programmatic of gene delivery to the blood-brain barrier. Pharm Res 27:2528-43 (2010). IHC-FoFr ; Mouse . Read more (PubMed: 20824308) »
See all 2 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab69313.
Please use the links above to contact us or submit feedback about this product.

For licensing inquiries, please contact partnerships@abcam.com

Sign up