Immunoprecipitation abreview for Anti-GFP antibody

Excellent
Abreviews
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HEK 293 cells transfected with Annexin1-GFP)
Total protein in input
25 µg
Specification
HEK 293 cells transfected with Annexin1-GFP
Immuno-precipitation step
Protein G

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% TX-100, 10% glycerol, 1X PBS

Western Blot antibody

Name
Non-Abcam antibody was used: Goat anti rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Additional data

Additional Notes
Line 1: Lysate of HEK 293 cells expressing Annexin1-GFP fusion
Line 2: IP with anti GFP Ab
Line 3: Not bound fraction

Herr Dr. Vladimir Milenkovic

Verified customer

Submitted Nov 16 2009

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