Immunohistochemistry - Free Floating abreview for Anti-GFP antibody

Excellent
Abreviews
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Brain)
Specification
Brain

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS+ 0.25% Triton-X

Secondary antibody

Name
Non-Abcam antibody was used: Dnk anti-Rb IgG (H+L)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy2 ®
Dilution
1/200

Additional data

Additional Notes
Image shows anti-NeuN (red), DAPI (blue), and anti-GFP staining of GFP-cre (green, yellow with NeuN colocalization). Free floating, 4% PFA-fixed, frozen 30 um sections were blocked for 1hr at RT with 10% normal donkey serum + Donkey anti-Mouse IgG Fab fragments (0.1 mg/ml). No antigen retrieval necessary.

Mrs. Judith Kranz

Verified customer

Submitted Jul 19 2010

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