Image shows anti-NeuN (red), DAPI (blue), and anti-GFP staining of GFP-cre (green, yellow with NeuN colocalization). Free floating, 4% PFA-fixed, frozen 30 um sections were blocked for 1hr at RT with 10% normal donkey serum + Donkey anti-Mouse IgG Fab fragments (0.1 mg/ml). No antigen retrieval necessary.
Mrs. Judith Kranz
Submitted Jul 19 2010
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