Immunoprecipitation abreview for Anti-GFP antibody

Excellent
Abreviews
Application
Immunoprecipitation
Sample
Human Cell lysate - nuclear (HEK 293 cells)
Total protein in input
20 µg
Specification
HEK 293 cells
Immuno-precipitation step
Protein G

Other product details

Concentration
1 µg/mg lysate
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: AFC (Low Salt) Buffer

Western Blot antibody

Name
Abcam antibody:Anti-GFP antibody
Dilution
1/5000

Additional data

Additional Notes
This was a control experiment for the IP protocol using cells expressing GFP. The control is cells with no GFP.
The image on your two Abreviews (25001 and 25002) is identical because both the IP and Western was done using the ab290 antibody (IP to pull down GFP and western to confirm the presence of GFP, which is a very common practice).

Mr. William Hung

Verified customer

Submitted Jul 18 2011

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