Western blot abreview for Anti-GFP antibody - ChIP Grade

Good
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (LNCaP cells)
Specification
LNCaP cells
Treatment
pEGFP-PKD1 transfected
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/5000

Secondary antibody

Name
Non-Abcam antibody was used: goat anti-rabbit secondary antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL
Bands
Specific: 130 kDa
Exposure
10 second(s)

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Submitted Feb 13 2015

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