Flow Cytometry abreview for Anti-GFP antibody - ChIP Grade

Excellent
Abreviews
Application
Flow Cytometry
Sample
Human Cell (Differentiated hNSCs)
Permeabilization
Yes - 0.25% Triton X-100 in DPBS
Gating Strategy
Undifferentiated Stem Cells (white)
Specification
Differentiated hNSCs
Preparation
Cell harvesting/tissue preparation method: Accutase
Sample buffer: PBS
Fixation
Paraformaldehyde

Other product details

Incubation time
20 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1% BSA in PBST
Dilution
1/300

Secondary antibody

Dilution
1/500
Name
Non-Abcam antibody was used: Alexa Fluor© 488 goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
These were transfected by nucleofection into the stem cells at the rosette stage of differentiation.GFP expression is expressed from the activation of the pan-neural marker Nestin and a non-transfected control stained with the Nestin antibody was used to validate the GFP expression.

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Submitted Aug 20 2015

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