Western blot abreview for Anti-GFP antibody - ChIP Grade

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (hek293)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
40 µg
Treatment
transfected with GFP-cDNA
Specification
hek293
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/2000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: fluorescent secondary
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 790

Detection

Detection method
Fluorescense
Exposure
1 hour(s), 0 minute(s) and 0 second(s)
Bands
Specific: 70, 100, 120 kDa
Positive control
GFP-fused cDNAs at various sizes

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Submitted Jun 12 2018

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