Western blot abreview for Anti-GFP antibody

Excellent
Abreviews
Application
Western blot
Sample
African Green Monkey Cell lysate - whole cell (Cos7 cells)
Loading amount
20 µg
Specification
Cos7 cells
Treatment
Transfected with either GFP or GFP-Eml4
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Dilution
1/5000
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS containing 2% milk and 1% tween

Secondary antibody

Name
Non-Abcam antibody was used: Swine-anti-Rabbit-HRP (DAKO)
Host species: Pig
Clonality: Polyclonal
Dilution
1/5000

Detection

Detection method
ECL
Exposure
10 second(s)
Bands
Specific: 30, 150 (Eml4 ~ 120) kDa

Additional data

PubMed ID
17196341: View

Dr. S Houtman

Verified customer

Submitted Jun 19 2007

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