Western blot abreview for Anti-GFP antibody [E385] (HRP)

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
10 µg
Specification
HEK293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 5% NFDM/TBST
Dilution
1/1000

Secondary antibody

Secondary antibody
None used

Detection

Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 60 kDa

Dr. Lin Zhu

Verified customer

Submitted Sep 08 2017

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