Western blot abreview for Anti-GFP antibody [EPR14104]

Excellent
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (various mouse tissue lysate: skeletal muscle, hear)
Gel Running Conditions
Non-reduced Denaturing (10% gel)
Loading amount
40 µg
Specification
various mouse tissue lysate: skeletal muscle, hear
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000

Secondary antibody

Dilution
1/3000
Name
Non-Abcam antibody was used: Amersham ECL Rabbit IgG, HRP-linked whole Ab
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Negative control
other tissues without expression the GFP-tagged protein. a-tubulin served as loading control.
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 30 kDa
Positive control
Transgenic mice heart tissue lysate overexpressed the GFP-tagged protein around 30 kDa

Additional data

Additional Notes
specific band. Good!

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Submitted Jun 19 2017

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