Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GFP antibody [EPR14104]

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (mouse tissue section (skin))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Permeabilization
Yes - 0.3% triton in PBS
Specification
mouse tissue section (skin)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking:5% Donkey serum, 1% BSA, 2% Col
Dilution
1/100

Secondary antibody

Dilution
1/400
Name
Non-Abcam antibody was used: AffiniPure Donkey Anti-Rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 488

Additional data

Additional Notes
15 micron paraffin sections were used for the staining. This antibody was also tried on thick 50 micron frozen sections. Using 0.3 triton in PBS for permeabilization, the antibody was diluted 1:50 in same blocking buffer described here, using these conditions the result was below average.

Dr. Yulia Shwartz

Verified customer

Submitted Aug 17 2017

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