Non-Abcam antibody was used: Cy™3 AffiniPure Fab Fragment Donkey Anti-Rabbit Ig Host species: Donkey Clonality: Polyclonal Conjugation: Cy3
Spinal cord tissue which was fixed in 4% PFA 4C 24Hr was cryoprotected and cut on a cryostat (60um) and processed free floating. The antibody labelled EGFP within the tissue (Advillin-GFP transgenic mouse from Gensat) consistent with images acquired of the native EGFP.
Submitted Oct 27 2017
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