Immunohistochemistry free floating abreview for Anti-GFP antibody [EPR14104]

Excellent
Abreviews
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Spinal Cord Dorsal Horn)
Specification
Spinal Cord Dorsal Horn

Other product details

Incubation time
72 hour(s) and 0 minute(s) · Temperature: 20°C
Dilution
1/117

Secondary antibody

Dilution
1/400
Name
Non-Abcam antibody was used: Cy™3 AffiniPure Fab Fragment Donkey Anti-Rabbit Ig
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ­

Additional data

Additional Notes
Spinal cord tissue which was fixed in 4% PFA 4C 24Hr was cryoprotected and cut on a cryostat (60um) and processed free floating. The antibody labelled EGFP within the tissue (Advillin-GFP transgenic mouse from Gensat) consistent with images acquired of the native EGFP.

Steven West

Verified customer

Submitted Oct 27 2017

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