Western blot abreview for Anti-GFP antibody [EPR14104]

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS)
Gel Running Conditions
Reduced Denaturing
Loading amount
15 µg
Specification
U2OS
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBST
Dilution
1/10000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: Anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW

Detection

Detection method
Fluorescence
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 130 kDa

Additional data

Additional Notes
Expressed GFP tagged NUP153 in U2OS cells and probed with anti-GFP on western blot

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Submitted Jan 28 2019

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