Product nameAnti-GFP antibody [GF28R]
See all GFP primary antibodies
DescriptionMouse monoclonal [GF28R] to GFP
Tested applicationsSuitable for: WB, ELISA, IP, Dot blot, ICC/IFmore details
Synthetic peptide corresponding to Aequorea victoria GFP (N terminal) conjugated to keyhole limpet haemocyanin.
Database link: P42212
- HEK293 cells transfected with GFP-tagged protein vector.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab127417 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/3000. Predicted molecular weight: 27 kDa.|
|ELISA||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|Dot blot||Use at an assay dependent concentration.|
|ICC/IF||1/500 - 1/2000.|
RelevanceFunction: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+ -activated photoprotein aequorin.
Subunit structure: Monomer.
Tissue specificity: Photocytes.
Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.
Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.
Sequence similarities: Belongs to the GFP family.
Biophysicochemical properties: Absorption: Abs(max)=395 nm
Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm.
- GFP antibody
- Green fluorescent protein antibody
All lanes : Anti-GFP antibody [GF28R] (ab127417) at 1/1000 dilution
Lane 1 : HEK293 cell lysate, non-transfected
Lane 2 : HEK293 cell lysate, transfected with GFP-tagged protein vector at 1 µg
Lane 3 : HEK293 cell lysate, transfected with GFP-tagged protein vector at 10 µg
Developed using the ECL technique.
Predicted band size: 27 kDa
This product has been referenced in:
- Nevzorov I et al. Myosin-1C uses a novel phosphoinositide-dependent pathway for nuclear localization. EMBO Rep 19:290-304 (2018). Read more (PubMed: 29330316) »
- Campa CC et al. Rab11 activity and PtdIns(3)P turnover removes recycling cargo from endosomes. Nat Chem Biol N/A:N/A (2018). Read more (PubMed: 29915378) »