Overview

  • Product name
    Anti-GFP antibody [GF28R]
    See all GFP primary antibodies
  • Description
    Mouse monoclonal [GF28R] to GFP
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ELISA, IP, Dot blot, ICC/IFmore details
  • Immunogen

    Synthetic peptide corresponding to Aequorea victoria GFP (N terminal) conjugated to keyhole limpet haemocyanin.
    Database link: P42212

  • Positive control
    • HEK293 cells transfected with GFP-tagged protein vector.
  • General notes
    ab127417 is stable for one year when stored properly.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Clonality
    Monoclonal
  • Clone number
    GF28R
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab127417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/3000. Predicted molecular weight: 27 kDa.
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
ICC/IF 1/500 - 1/2000.

Target

  • Relevance
    Function: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+ -activated photoprotein aequorin.

    Subunit structure: Monomer.

    Tissue specificity: Photocytes.

    Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

    Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.

    Sequence similarities: Belongs to the GFP family.

    Biophysicochemical properties: Absorption: Abs(max)=395 nm
    Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm.
  • Alternative names
    • GFP antibody
    • Green fluorescent protein antibody

Images

  • All lanes : Anti-GFP antibody [GF28R] (ab127417) at 1/1000 dilution

    Lane 1 : HEK293 cell lysate, non-transfected
    Lane 2 : HEK293 cell lysate, transfected with GFP-tagged protein vector at 1 µg
    Lane 3 : HEK293 cell lysate, transfected with GFP-tagged protein vector at 10 µg

    Developed using the ECL technique.

    Predicted band size: 27 kDa

References

This product has been referenced in:
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (human)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Treatment
alpha synuclein for 24hrs
Specification
human
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C

Ms. Sehee Oh

Verified customer

Submitted Jul 13 2016

Question
Answer

We have not specifically tested d1/d2EGFP but beleive that ab127417 will be able to detect this. We also recommend using out anti-EGFP antibody ab111258 as we have been informed that this will work as well.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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