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resp. sir can we use the same antibody for staining mammalian brain sections(including human), and that too on perfusion fixed(excluding human) cryosections using PAP protocol
Asked on Jun 23 2001
We have been unable to find any information on whether the estrogen receptor is expressed in brain - this antibody was primarily produced for the diagnosis of breast tumours. Secondly, although this antibody can be used in frozen (cryostat) sections, we do not know whether it recognises the antigen in species other than human. Nevertheless, the PAP staining method should be fine.
Neil HaywardAbcam Scientific Support
Answered on Jun 25 2001