Human Cell lysate - whole cell (Hela, Huh7 and 293 cells)
Hela, Huh7 and 293 cells
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris Gel)
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
1 hour(s) and 0 minute(s) · Temperature: 24°C
Non-Abcam antibody was used: ECL Mouse IgG, HRP-Linked Whole Ab Host species: Sheep Clonality: Monoclonal Conjugation: Horse Radish Peroxidase
Specific: 27 kDa
CMV-GFP plasmid was used as a transfection control and the GFP expression was confirmed using fluorescent microscope. For Western GFP was used to normalize the expression of another protein of interest. Thus all samples were GFP+ and were controls for themselves
untransduced cells were used as negative control.
I used the antibody at lower concentration than recommended by the Abcam. They suggest 0.5ug/ml and I used 0.2ug/ml. It worked very well at that concentration.
Dr. Leszek Lisowski
Submitted Jan 06 2009
Get resources and offers direct to your inboxSign up