Immunoprecipitation abreview for Anti-GFP antibody [LGB-1]

Good
Abreviews
Application
Immunoprecipitation
Sample
Human Cell lysate - other (HEK 293)
Total protein in input
300 µg
Specification
HEK 293
Treatment
Transfected with CFP-fused protein XXX in pECFP vector
Immuno-precipitation step
Protein G

Other product details

Concentration
6.7 µg/mg lysate
Incubation time
30 minute(s) · Temperature: 4°C · Diluent: PBS + detergent + protease inhibitors

Western Blot antibody

Western Blot antibody
None used

Additional data

Additional Notes
ab291 rapidly bound CFP (depleted from 'unbound' fraction compared to total lysate) allowing IP of CFP-fused protein XXX in as little as 30 min. 2ug captured almost all of the target protein from only 300ug total protein in an overnight IP: a lot of ab291 antibody may be required for complete IP.

Dr. Lindsay Tulloch

Verified customer

Submitted Dec 10 2009

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