Immunocytochemistry/ Immunofluorescence abreview for Anti-GFP antibody [LGB-1]

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Dog Cell (MDCK II cells transfected with GFP)
Specification
MDCK II cells transfected with GFP
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% TX100
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5%

Other product details

Dilution
1/250
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.1% TX100, 1% goat serum, 1X PBS

Secondary antibody

Name
Non-Abcam antibody was used: anti mouse 546
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 546
Dilution
1/500

Additional data

Additional Notes
GFP antibody (ab291) was used to assess electroporation efficiency of double transfected MDCK II cells. DAPI was used to stain nuclei.

Herr Dr. Vladimir Milenkovic

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Submitted Jun 16 2010

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