Western blot abreview for Anti-GFP antibody [LGB-1]

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK 293 cells transfected with Rab27a-GFP)
Loading amount
25 µg
Specification
HEK 293 cells transfected with Rab27a-GFP
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1XPBS-Tween, 5% milk

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti mouse HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL+
Exposure
15 minute(s) and 0 second(s)
Bands
Specific: 55 kDa

Herr Dr. Vladimir Milenkovic

Verified customer

Submitted Jul 22 2010

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