Embryos were electroporated with Plasmid 13776: pCAG-Cre:GFP from Addgene and then fixed with 4% PFA overnight at 4 degree. Samples were embedded in gelatin and sectioned using a cryostat. Left image is DAPI plus GFP (without staining). Right images is using anti-GFP antibody [LGB-1] (ab291) at 1:200 dilution, the secondary antibody used was donkey anti-mouse Alexa 568 from invitrogen (1:1000).
GFP can be detected using anti-GFP ab13970, but not anti-GFP ab291.
Thank you for sharing this data with us. Previously ab291 has not been tested in IHC-Fr and based on this abreview, it looks like this antibody is not suitable for IHC-Fr under these conditions. Abcam would welcome feedback from other customers currently using this product.
Abcam user community
Submitted Mar 20 2014
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