GFP ELISA Kit (ab171581)
- Datasheet
- References (6)
- Protocols
Overview
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Product name
GFP ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Cell Lysate 9 3.1% Inter-assay Sample n Mean SD CV% Cell Lysate 3 8.2% -
Sample type
Cell Lysate, Cell culture media, Tissue Lysate -
Assay type
Sandwich (quantitative) -
Sensitivity
1.8 pg/ml -
Range
2.7 pg/ml - 2000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture media 102 101% - 103% Fetal Bovine Serum 97 96% - 98% -
Assay time
1h 30m -
Assay duration
One step assay -
Product overview
GFP ELISA kit (ab171581) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GFP protein in cell and tissue lysates. It uses our proprietary SimpleStep ELISA® technology. Quantitate human GFP with 1.8 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.
This kit will detect both the Enhanced and Superfold variants of GFP.
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Notes
Green fluorescent protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technology has considerably contributed to a greater understanding of cellular physiology.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X GFP Capture Antibody 1 x 600µl 10X GFP Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 4X Antibody Diluent EB 1 x 6ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml GFP Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 x 96 tests Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+-activated photoprotein aequorin. -
Alternative names
- Green Flourescent Protein
Images
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Other - GFP ELISA Kit (ab171581)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example GFP standard curveBackground-subtracted data values (mean +/- SD) are graphed.
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Example of raw data for GFP standard curveRaw data values are shown in the table
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GFP cross reactivity dataThis kit recognizes both GFP and enhanced GFP (eGFP) in cell and tissue extracts. No reactivity with mCherry, RFP or YFP was observed.
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Titration of GFP spiked 143B cell lysate within the working range of the assayBackground subtracted data from triplicate measurements are plotted.
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Linearity of dilution for spiked GFPGFP spiked 143B lysate was measured in 3-fold dilution series
Protocols
References
This product has been referenced in:
- Lin CJ et al. Microbubble-facilitated ultrasound pulsation promotes direct a-synuclein gene delivery. Biochem Biophys Res Commun 517:77-83 (2019). Read more (PubMed: 31327496) »
- Yu K et al. Proof of principle: Physiological transfer of small numbers of bacteria from mother to fetus in late-gestation pregnant sheep. PLoS One 14:e0217211 (2019). Read more (PubMed: 31170184) »
