GFP ELISA Kit (ab171581)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2.8 pg/ml
- Range: 18.8 pg/ml - 1200 pg/ml
- Sample type: Cell culture media, Cell Lysate, Tissue Lysate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
Overview
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Product name
GFP ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Cell Lysate 9 3.1% Inter-assay Sample n Mean SD CV% Cell Lysate 3 8.2% -
Sample type
Cell Lysate, Cell culture media, Tissue Lysate -
Assay type
Sandwich (quantitative) -
Sensitivity
2.8 pg/ml -
Range
18.8 pg/ml - 1200 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture media 102 101% - 103% Fetal Bovine Serum 97 96% - 98% -
Assay time
1h 30m -
Assay duration
One step assay -
Product overview
GFP ELISA kit (ab171581) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GFP protein in cell and tissue lysates. It uses our proprietary SimpleStep ELISA® technology. Quantitate human GFP with 1.8 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
Watch our short animated video to see how it works
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.
This kit will detect both the Enhanced and Superfold variants of GFP.
Accurate ELISAs don't have to take 3 hours or more. SimpleStep ELISA® kits deliver fast results in just 90 minutes by reducing antibody and sample additions to a single step.
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Notes
Green fluorescent protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technology has considerably contributed to a greater understanding of cellular physiology.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X GFP Capture Antibody 1 x 600µl 10X GFP Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 4X Antibody Diluent EB 1 x 6ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml GFP Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+-activated photoprotein aequorin. -
Alternative names
- Green Flourescent Protein
Images
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Other - GFP ELISA Kit (ab171581)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example GFP standard curveBackground-subtracted data values (mean +/- SD) are graphed.
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Example of raw data for GFP standard curveRaw data values are shown in the table
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GFP cross reactivity dataThis kit recognizes both GFP and enhanced GFP (eGFP) in cell and tissue extracts. No reactivity with mCherry, RFP or YFP was observed.
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Titration of GFP spiked 143B cell lysate within the working range of the assayBackground subtracted data from triplicate measurements are plotted.
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AbReviewThis image is courtesy submitted by Ling ChunThe kit works with superfold GFP.
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Linearity of dilution for spiked GFPGFP spiked 143B lysate was measured in 3-fold dilution series
Datasheets and documents
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SDS download
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Datasheet download
References (14)
ab171581 has been referenced in 14 publications.
- Yu K et al. Transfer of oral bacteria to the fetus during late gestation. Sci Rep 11:708 (2021). PubMed: 33436911
- Ding K et al. Proteosomal degradation impairs transcytosis of AAV vectors from suprachoroidal space to retina. Gene Ther N/A:N/A (2021). PubMed: 33542456
- Anjo SI et al. A different vision of translational research in biomarker discovery: a pilot study on circulatory mitochondrial proteins as Parkinson's disease potential biomarkers. Transl Neurodegener 9:11 (2020). PubMed: 32266064
- Wesslowski J et al. eGFP-tagged Wnt-3a enables functional analysis of Wnt trafficking and signaling and kinetic assessment of Wnt binding to full-length Frizzled. J Biol Chem 295:8759-8774 (2020). PubMed: 32381507
- Frederick A et al. Engineered Capsids for Efficient Gene Delivery to the Retina and Cornea. Hum Gene Ther 31:756-774 (2020). PubMed: 32578442