Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell culture extracts, Cell Lysate, Tissue Extracts, Tissue Homogenate, Tissue Lysate
Product nameGFP Quantification Kit
See all GFP kits
Sample typeCell culture extracts, Tissue Extracts, Cell Lysate, Tissue Homogenate, Tissue Lysate
Range0.01 µg/ml - 10 µg/ml
The GFP Quantification Kit (ab235672) quantifies GFP in a 96 microplate format for a wide array of cells and tissues.
This kit can detect a wide range of GFP concentrations (0.01-10 μg/ml). Cells or tissues can be homogenized directly in the GFP Assay Buffer, the quantity of GFP is then determined by comparing the fluorescence with that of the GFP standard. Ex/Em = 488/507 nm.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests GFP Assay Buffer 1 x 25ml GFP Quench Solution 1 x 1ml GFP Standard (100 µg) 1 vial
RelevanceFunction: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+ -activated photoprotein aequorin.
Subunit structure: Monomer.
Tissue specificity: Photocytes.
Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.
Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.
Sequence similarities: Belongs to the GFP family.
Biophysicochemical properties: Absorption: Abs(max)=395 nm
Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm.
- Green fluorescent protein
ab235672 has not yet been referenced specifically in any publications.