Overview

  • Product name

    GFP Quantification Kit
    See all GFP kits
  • Detection method

    Fluorescent
  • Sample type

    Cell culture extracts, Tissue Extracts, Cell Lysate, Tissue Homogenate, Tissue Lysate
  • Assay type

    Quantitative
  • Range

    0.01 µg/ml - 10 µg/ml
  • Product overview

    The GFP Quantification Kit (ab235672) quantifies GFP in a 96 microplate format for a wide array of cells and tissues.


    This kit can detect a wide range of GFP concentrations (0.01-10 μg/ml). Cells or tissues can be homogenized directly in the GFP Assay Buffer, the quantity of GFP is then determined by comparing the fluorescence with that of the GFP standard. Ex/Em = 488/507 nm.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    GFP Assay Buffer 1 x 25ml
    GFP Quench Solution 1 x 1ml
    GFP Standard  (100 µg) 1 vial
  • Research areas

  • Relevance

    Function: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+ -activated photoprotein aequorin.

    Subunit structure: Monomer.

    Tissue specificity: Photocytes.

    Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

    Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.

    Sequence similarities: Belongs to the GFP family.

    Biophysicochemical properties: Absorption: Abs(max)=395 nm
    Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm.
  • Alternative names

    • GFP
    • Green fluorescent protein

Images

  • GFP standard curve.

Protocols

References

ab235672 has not yet been referenced specifically in any publications.

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