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    gfp-quantification-kit-ab235672.pdf

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Tags & Cell Markers Fusion / Marker Proteins GFP
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GFP Quantification Kit (ab235672)

  • Datasheet
  • SDS
  • Protocol Booklet
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Example Data

    Key features and details

    • Assay type: Quantitative
    • Detection method: Fluorescent
    • Platform: Microplate reader
    • Sample type: Cell culture extracts, Cell Lysate, Tissue Extracts, Tissue Homogenate, Tissue Lysate

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    Overview

    • Product name

      GFP Quantification Kit
      See all GFP kits
    • Detection method

      Fluorescent
    • Sample type

      Cell culture extracts, Tissue Extracts, Cell Lysate, Tissue Homogenate, Tissue Lysate
    • Assay type

      Quantitative
    • Range

      0.01 µg/ml - 10 µg/ml
    • Product overview

      The GFP Quantification Kit (ab235672) quantifies GFP in a 96 microplate format for a wide array of cells and tissues.


      This kit can detect a wide range of GFP concentrations (0.01-10 μg/ml). Cells or tissues can be homogenized directly in the GFP Assay Buffer, the quantity of GFP is then determined by comparing the fluorescence with that of the GFP standard. Ex/Em = 488/507 nm.

    • Notes

      This product is manufactured by BioVision, an Abcam company and was previously called K815 GFP Quantitation Kit. K815-100 is the same size as the 100 test size of ab235672.

    • Platform

      Microplate reader

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 100 tests
      GFP Assay Buffer 1 x 25ml
      GFP Quench Solution 1 x 1ml
      GFP Standard  (100 µg) 1 vial
    • Research areas

      • Tags & Cell Markers
      • Fusion / Marker Proteins
      • GFP
    • Relevance

      Function: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+ -activated photoprotein aequorin.

      Subunit structure: Monomer.

      Tissue specificity: Photocytes.

      Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

      Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.

      Sequence similarities: Belongs to the GFP family.

      Biophysicochemical properties: Absorption: Abs(max)=395 nm
      Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm.
    • Alternative names

      • GFP
      • Green fluorescent protein

    Images

    • Example Data
      Example Data

      GFP standard curve.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab235672? Please let us know so that we can cite the reference in this datasheet.

    ab235672 has been referenced in 1 publication.

    • Poudel S  et al. Oral Delivery of Nucleic Acids with Passive and Active Targeting to the Intestinal Tissue Using Polymer-Based Nanocarriers. Pharmaceutics 13:N/A (2021). PubMed: 34371766

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