Recombinant

Recombinant Anti-GFP VHH Single Domain antibody (Alexa Fluor® 488) (ab192863)

Overview

  • Product name

    Anti-GFP VHH Single Domain antibody (Alexa Fluor® 488)
    See all GFP VHH Single Domain primary antibodies
  • Description

    Llama monoclonal to GFP VHH Single Domain (Alexa Fluor® 488)
  • Host species

    Llama
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Specificity

    Specifically binds GFP, eGFP and BFP. ab192863 binds GFP N-terminal and C-terminal fusion proteins. It does not interact with CFP, RFP, YFP, mCherry or TurboGFP.
  • Tested applications

    Suitable for: ICC/IFmore details
  • Immunogen

    Recombinant full length protein corresponding to Aequorea victoria GFP VHH Single Domain.

  • Positive control

    • This antibody gave a positive signal in NIH3T3 overexpressing GFP cell line within IP.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituents: PBS, Sodium chloride, Sodium phosphate, 30% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    This product is a recombinant protein produced in E. coli.
  • Clonality

    Monoclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab192863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance

    Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+-activated photoprotein aequorin. Subunit structure: Monomer. Tissue specificity: Photocytes. Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen. Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy. Sequence similarities: Belongs to the GFP family. Absorption: Abs(max)=395 nm. Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm.
  • Alternative names

    • GFP antibody
    • Green fluorescent protein antibody
    • yfp antibody

Images

  • Anti-GFP VHH Single Domain Antibody (Alexa Fluor® 488) (ab192863) staining GFP in GFP-transfected NIH3T3 cells. The cells were fixed with 4% formaldehyde (10min) and then blocked in 1% BSA / 0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab192863, at 0.1μg/ml overnight at +4°C (green). Nuclear DNA was labelled with 1.43μM DAPI (blue).

    Under identical experimental conditions, when compared to the basal level of GFP expression in transfected NIH3T3 cells, the cells upon which ab192863 was applied gave a stronger signal in the 488 channel, indicating that ab192863 is binding to GFP and therefore eliciting signal amplification.

    Ab192863 was also applied to non-GFP-transfected NIH3T3 cells, which produced no positive staining, indicating specificity for GFP.

References

ab192863 has not yet been referenced specifically in any publications.

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