Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Llama monoclonal to GFP VHH Single Domain (Alexa Fluor® 488)
- Suitable for: ICC/IF
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Product nameAnti-GFP VHH Single Domain antibody (Alexa Fluor® 488)
See all GFP VHH Single Domain primary antibodies
DescriptionLlama monoclonal to GFP VHH Single Domain (Alexa Fluor® 488)
ConjugationAlexa Fluor® 488. Ex: 495nm, Em: 519nm
SpecificitySpecifically binds GFP, eGFP and BFP. ab192863 binds GFP N-terminal and C-terminal fusion proteins. It does not interact with CFP, RFP, YFP, mCherry or TurboGFP.
Tested applicationsSuitable for: ICC/IFmore details
Recombinant full length protein corresponding to Aequorea victoria GFP VHH Single Domain.
- This antibody gave a positive signal in NIH3T3 overexpressing GFP cell line within IP.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or email@example.com.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, Sodium chloride, Sodium phosphate, 30% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
Purification notesThis product is a recombinant protein produced in E. coli.
Our Abpromise guarantee covers the use of ab192863 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
RelevanceEnergy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+-activated photoprotein aequorin. Subunit structure: Monomer. Tissue specificity: Photocytes. Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen. Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy. Sequence similarities: Belongs to the GFP family. Absorption: Abs(max)=395 nm. Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm.
- GFP antibody
- Green fluorescent protein antibody
- yfp antibody
Anti-GFP VHH Single Domain Antibody (Alexa Fluor® 488) (ab192863) staining GFP in GFP-transfected NIH3T3 cells. The cells were fixed with 4% formaldehyde (10min) and then blocked in 1% BSA / 0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab192863, at 0.1μg/ml overnight at +4°C (green). Nuclear DNA was labelled with 1.43μM DAPI (blue).
Under identical experimental conditions, when compared to the basal level of GFP expression in transfected NIH3T3 cells, the cells upon which ab192863 was applied gave a stronger signal in the 488 channel, indicating that ab192863 is binding to GFP and therefore eliciting signal amplification.
Ab192863 was also applied to non-GFP-transfected NIH3T3 cells, which produced no positive staining, indicating specificity for GFP.
ab192863 has not yet been referenced specifically in any publications.