Key features and details
- Rabbit polyclonal to GFPP
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GFPP antibody
See all GFPP primary antibodies
DescriptionRabbit polyclonal to GFPP
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Rhesus monkey
Recombinant fragment within Human GFPP (internal sequence). The exact sequence is proprietary.
Database link: O14772
- WB: A431 whole cell lysate. IHC-P: Human colon carcinoma tissue. ICC/IF: A431 cells
This product was previously labelled as FPGT
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229236 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 67 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionCatalyzes the formation of GDP-L-fucose from GTP and L-fucose-1-phosphate. Functions as a salvage pathway to reutilize L-fucose arising from the turnover of glycoproteins and glycolipids.
Tissue specificityExpressed in many tissues.
- Information by UniProt
- FPGT antibody
- FPGT_HUMAN antibody
- Fucose 1 phosphate guanyltransferase antibody
4% paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells stained for GFPP (green) using ab229236 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
Paraffin-embedded human colon carcinoma tissue stained for GFPP using ab229236 at 1/500 dilution in immunohistochemical analysis.
Anti-GFPP antibody (ab229236) at 1/2000 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 67 kDa
7.5% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229236 has not yet been referenced specifically in any publications.