Overview

  • Product name

    Anti-GFPT1 antibody [EPR4854]
    See all GFPT1 primary antibodies
  • Description

    Rabbit monoclonal [EPR4854] to GFPT1
  • Host species

    Rabbit
  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human GFPT1 aa 600-700. The exact sequence is proprietary.

  • Positive control

    • IHC-P: Human testis, and Human breast carcinoma tissue; WB: Human placenta lysate, MCF7, C6, MEF, 293T, JAR and HeLa cell line lysates, Mouse heart and Mouse cerebral cortex, Rat heart tissue lysate. IP: Jurkat cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab125069 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa).

For unpurified use at 1/1000 - 1/1000.

IP 1/10 - 1/100.
IHC-P 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified use at 1/100 - 1/250.

See IHC antigen retrieval protocols.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function

      Controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins.
    • Tissue specificity

      Isoform 1 is predominantly expressed in skeletal muscle. Not expressed in brain. Seems to be selectively expressed in striated muscle.
    • Pathway

      Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate: step 1/1.
    • Involvement in disease

      Defects in GFPT1 are the cause of limb-girdle myasthenia with tubular aggregates (LGMTA) [MIM:610542]. A congenital myasthenic syndrome characterized by onset of proximal muscle weakness in the first decade. Individuals with this condition have a recognizable pattern of weakness of shoulder and pelvic girdle muscles, and sparing of ocular or facial muscles. EMG classically shows a decremental response to repeated nerve stimulation, a sign of neuromuscular junction dysfunction. Affected individuals show a favorable response to acetylcholinesterase (AChE) inhibitors.
    • Sequence similarities

      Contains 1 glutamine amidotransferase type-2 domain.
      Contains 2 SIS domains.
    • Information by UniProt
    • Database links

    • Alternative names

      • CMS12 antibody
      • CMSTA1 antibody
      • D-fructose-6-phosphate amidotransferase 1 antibody
      • GFA antibody
      • GFAT 1 antibody
      • GFAT antibody
      • GFAT1 antibody
      • GFAT1m antibody
      • GFPT antibody
      • Gfpt1 antibody
      • GFPT1_HUMAN antibody
      • GFPT1L antibody
      • Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] 1 antibody
      • Glutamine--fructose-6-phosphate transaminase 1 antibody
      • Glutamine:fructose 6 phosphate amidotransferase 1 antibody
      • Hexosephosphate aminotransferase 1 antibody
      • MSLG antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling GFPT1 with Purified ab125069 at 1:1000 dilution (2.3 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • ab125069 (purified) at 1:100 dilution (2µg) immunoprecipitating GFPT1 in Jurkat whole cell lysate.
      Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10µg
      Lane 2 (+): ab125069 & Jurkat whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab125069 in Jurkat whole cell lysate
      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

    • Anti-GFPT1 antibody [EPR4854] (ab125069) at 1/200 dilution (Purified) + C6 (Rat glial tumor glial cell) whole cell lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 79 kDa
      Observed band size: 79 kDa

    • All lanes : Anti-GFPT1 antibody [EPR4854] (ab125069) at 1/1000 dilution (Purified)

      Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
      Lane 2 : Mouse heart lysates
      Lane 3 : Mouse cerebral cortex lysates
      Lane 4 : MEF (Mouse embryonic fibroblast (immortalized)) whole cell lysates
      Lane 5 : Rat heart lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 79 kDa
      Observed band size: 79 kDa



      This antibody is not suitable to detect tissue lysate samples.

    • ab125069, at a 1/100 dilution, staining GFPT1 in paraffin-embedded Human testis tissue by immunohistochemistry.
    • All lanes : Anti-GFPT1 antibody [EPR4854] (ab125069) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : JAR cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : Human placenta lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 79 kDa

    References

    This product has been referenced in:

    • Shi H  et al. Skeletal muscle O-GlcNAc transferase is important for muscle energy homeostasis and whole-body insulin sensitivity. Mol Metab N/A:N/A (2018). Read more (PubMed: 29525407) »
    • Kavanagh Williamson M  et al. Upregulation of Glucose Uptake and Hexokinase Activity of Primary Human CD4+ T Cells in Response to Infection with HIV-1. Viruses 10:N/A (2018). Read more (PubMed: 29518929) »
    See all 7 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    25 µg
    Specification
    HeLa
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

    Abcam user community

    Verified customer

    Submitted Jan 12 2018

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HBEC cells)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    20 µg
    Treatment
    glucose deprivation
    Specification
    HBEC cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Jun 08 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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