Recombinant
RabMAb

Recombinant Anti-GFPT2 antibody [EPR19095] (Alexa Fluor® 488) (ab215948)

Overview

  • Product name

    Anti-GFPT2 antibody [EPR19095] (Alexa Fluor® 488)
    See all GFPT2 primary antibodies
  • Description

    Rabbit monoclonal [EPR19095] to GFPT2 (Alexa Fluor® 488)
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Human GFPT2 aa 1-300. The exact sequence is proprietary.
    Database link: O94808

  • Positive control

    • ICC/IF: U87MG cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab215948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

This product gave a positive signal in U87MG cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)

Target

  • Function

    Controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins.
  • Tissue specificity

    Highest levels of expression in heart, placenta, and spinal cord.
  • Pathway

    Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate: step 1/1.
  • Sequence similarities

    Contains 1 glutamine amidotransferase type-2 domain.
    Contains 2 SIS domains.
  • Information by UniProt
  • Database links

  • Alternative names

    • D fructose 6 phosphate amidotransferase 2 antibody
    • D-fructose-6-phosphate amidotransferase 2 antibody
    • GFAT 2 antibody
    • GFAT2 antibody
    • Gfpt2 antibody
    • GFPT2_HUMAN antibody
    • Glucosamine fructose 6 phosphate aminotransferase [isomerizing] 2 antibody
    • glutamine fructose 6 phosphate transaminase 2 antibody
    • Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2 antibody
    • Glutamine:fructose 6 phosphate amidotransferase 2 antibody
    • Glutamine:fructose-6-phosphate amidotransferase 2 antibody
    • Hexosephosphate aminotransferase 2 antibody
    see all

Images

  • ab215948 staining GFPT2 in U87MG cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab215948 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in U87MG cells fixed with 100% methanol (5 min).

References

ab215948 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab215948.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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