Overview

  • Product name

    Anti-GFR alpha 3/GFRA3 antibody
    See all GFR alpha 3/GFRA3 primary antibodies
  • Description

    Chicken polyclonal to GFR alpha 3/GFRA3
  • Host species

    Chicken
  • Specificity

    ab205361 doesn’t cross-react with hGFRa-1, hGFRa-2, hGFRa-4
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein (His-tag) corresponding to Human GFR alpha 3/GFRA3. Produced using CHO-based proprietary suspension cell line. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant.
    Database link: O60609

  • Positive control

    • U2OS cells; His tagged GFR alpha 3/GFRA3 protein.
  • General notes

     This product was previously labelled as GFR alpha 3

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Constituent: 100% PBS

    As product is (NH4)2SO4 precipitate, mix well by pipetting or vortexing prior use.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgY
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab205361 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 44 kDa.
ICC/IF Use a concentration of 0.3 - 0.67 µg/ml.

Target

  • Function

    Receptor for the glial cell line-derived neurotrophic factor, ARTN (artemin). Mediates the artemin-induced autophosphorylation and activation of the RET receptor tyrosine kinase.
  • Tissue specificity

    Widely expressed in adult and fetus which exhibit a similar pattern. Essentially not expressed in the central nervous system, but highly expressed in several sensory and sympathetic ganglia of the peripheral nervous system. Moderate expression in many non-neuronal tissues, particularly those of the digestive and urogenital systems, but high expression in stomach and appendix. Several types of glandular tissues show low expression. Very low or no expression detected in the hematopoietic system.
  • Sequence similarities

    Belongs to the GDNFR family.
  • Post-translational
    modifications

    N-glycosylated.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • GDNF family receptor alpha 3 antibody
    • GDNF family receptor alpha-3 antibody
    • GDNF receptor alpha-3 antibody
    • GDNFR-alpha-3 antibody
    • GDNFR3 antibody
    • GFR-alpha-3 antibody
    • GFRA3 antibody
    • GFRA3_HUMAN antibody
    • Glial cell line derived neurotrophic factor receptor alpha 3 antibody
    • GPI linked receptor antibody
    • OTTHUMP00000159486 antibody
    • OTTHUMP00000224020 antibody
    see all

Images

  • Immunofluorescent analysis of U2OS cells labeling GFR alpha 3/GFRA3 using ab205361 at 1/3000. Goat anti-chicken IgY - H&L DyLight 550 (ab96952) at 1/200 dilution was used as secondary antibody. For nuclear staining DAPI was used.

  • Anti-GFR alpha 3/GFRA3 antibody (ab205361) at 1/5000 dilution + His tagged GFR alpha 3/GFRA3 protein at 0.1 µg

    Secondary
    Rabbit anti-chicken antibody

    Predicted band size: 44 kDa

References

ab205361 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab205361.
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