Overview

  • Product name

    Anti-GFR alpha 3/GFRA3 antibody
    See all GFR alpha 3/GFRA3 primary antibodies
  • Description

    Rabbit polyclonal to GFR alpha 3/GFRA3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC (PFA fixed), IHC-P, IHC - Wholemount, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse GFR alpha 3/GFRA3 aa 347-360.
    (Peptide available as ab6100)

  • Positive control

    • Murine kidney, liver or heart tissue lysates.
  • General notes


    Members of the glial cell line-derived neurotrophic factor(GDNF) family including GDNF and neurturin (NTN) play essential roles in the control of vertebrate neuron survival and differentiation. A new member of the GDNF family was recently identified and designated persephin. Physiological responses to these neurotrophic factors require two receptor subunits, the novel glycosylphosphadidylinositol linked protein GFRa and Ret receptor tyrosine kinase GFRb. Following the findings of GFRa-1 and -2, a novel receptor in GFRa family was identified very recently from human and mouse and designated GFRa-3. GFRa-3 binds persephin, thus, persephin, GFRa-3, and Ret PTK form a complex to transduce persephin signal and to mediate persephin function.

     This product was previously labelled as GFR alpha 3

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • Purity

    DEAE-Chromatography
  • Primary antibody notes

    Members of the glial cell line-derived neurotrophic factor(GDNF) family including GDNF and neurturin (NTN) play essential roles in the control of vertebrate neuron survival and differentiation. A new member of the GDNF family was recently identified and designated persephin. Physiological responses to these neurotrophic factors require two receptor subunits, the novel glycosylphosphadidylinositol linked protein GFRa and Ret receptor tyrosine kinase GFRb. Following the findings of GFRa-1 and -2, a novel receptor in GFRa family was identified very recently from human and mouse and designated GFRa-3. GFRa-3 binds persephin, thus, persephin, GFRa-3, and Ret PTK form a complex to transduce persephin signal and to mediate persephin function.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC (PFA fixed) 1/50.
IHC-P Use a concentration of 10 µg/ml.
IHC - Wholemount Use at an assay dependent concentration. PubMed: 23152897
WB 1/500 - 1/1000. Predicted molecular weight: 43 kDa.Can be blocked with GFR alpha 3/GFRA3 peptide (ab6100).

Target

  • Function

    Receptor for the glial cell line-derived neurotrophic factor, ARTN (artemin). Mediates the artemin-induced autophosphorylation and activation of the RET receptor tyrosine kinase.
  • Tissue specificity

    Widely expressed in adult and fetus which exhibit a similar pattern. Essentially not expressed in the central nervous system, but highly expressed in several sensory and sympathetic ganglia of the peripheral nervous system. Moderate expression in many non-neuronal tissues, particularly those of the digestive and urogenital systems, but high expression in stomach and appendix. Several types of glandular tissues show low expression. Very low or no expression detected in the hematopoietic system.
  • Sequence similarities

    Belongs to the GDNFR family.
  • Post-translational
    modifications

    N-glycosylated.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • GDNF family receptor alpha 3 antibody
    • GDNF family receptor alpha-3 antibody
    • GDNF receptor alpha-3 antibody
    • GDNFR-alpha-3 antibody
    • GDNFR3 antibody
    • GFR-alpha-3 antibody
    • GFRA3 antibody
    • GFRA3_HUMAN antibody
    • Glial cell line derived neurotrophic factor receptor alpha 3 antibody
    • GPI linked receptor antibody
    • OTTHUMP00000159486 antibody
    • OTTHUMP00000224020 antibody
    see all

Images

  • All lanes : Anti-GFR alpha 3/GFRA3 antibody (ab8028) at 1/500 dilution

    Lane 1 : Crude cell membrane fractions of murine heart
    Lane 2 : Crude cell membrane fractions of murine spleen
    Lane 3 : Crude cell membrane fractions of murine kidney
    Lane 4 : Crude cell membrane fractions of murine liver
    Lane 5 : Crude cell membrane fractions of murine brain

    Predicted band size: 43 kDa
    Observed band size: 43 kDa



    Western blot analysis of GFR alpha 3/GFRA3 in crude cell membrane fractions of
    murine heart (H), spleen (S), kidney (K), liver (L), and brain (B), respectively, with ab8028 at 1/500 dilution.

  • Immunohistochemistry (Formalin-fixed paraffin embedded sections) of mouse kidney tissue labeling GFR alpha 3/GFRA3 with Anti-GFR alpha 3/GFRA3 antibody (ab8028) at 5μg/ml.

  • ab8028 at 10µg/ml staining GFR alpha 3/GFRA3 in mouse heart tissue by IHC-P.

  • Immunohistochemical analysis of cleavage-stage Human embryo, staining GFR alpha 3/GFRA3 (green) with ab8028.

    Embyros were fixed with 4% paraformaldehyde for 30 min at 23°C. After permeabilization with 0.1% Triton X-100, embryos were pre-incubated in 5% BSA for 1 hour before incubation with primary antibody (diluted in PBS with 1% BSA) overnight at 4°C. A FITC-conjugated anti-rabbit IgG was used as the secondary antibody.

References

This product has been referenced in:

  • Ito Y  et al. Identification of targets of tumor suppressor microRNA-34a using a reporter library system. Proc Natl Acad Sci U S A 114:3927-3932 (2017). Read more (PubMed: 28356515) »
  • Kawamura K  et al. Promotion of human early embryonic development and blastocyst outgrowth in vitro using autocrine/paracrine growth factors. PLoS One 7:e49328 (2012). IHC - Wholemount ; Human . Read more (PubMed: 23152897) »
See all 4 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Flow Cytometry
Sample
Mouse Cell (Dorsal Root Ganglion (DRG) Cells E12)
Permeabilization
Yes - Saponin
Gating Strategy
1. FSC-A/SSC-A (exclusion of cell debris), 2. SSC-W (exclusion of cell doublets)
Specification
Dorsal Root Ganglion (DRG) Cells E12
Preparation
Cell harvesting/tissue preparation method: Trypsinization. Trituration.
Sample buffer: PBS BSA 1%
Fixation
Paraformaldehyde

Georg Haase

Verified customer

Submitted Oct 14 2015

Answer

Thank you for your recent enquiry. Unfortunately, this antibody has not been tested for cross-reactivity with other family members. We apologise for the inconvenience caused, Please do not hesitate to contact us again if you need further information,

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