Anti-GFR alpha 3/GFRA3 antibody (ab8028)
Key features and details
- Rabbit polyclonal to GFR alpha 3/GFRA3
- Suitable for: IHC-P, WB
- Reacts with: Mouse
- Isotype: IgG
Overview
-
Product name
Anti-GFR alpha 3/GFRA3 antibody
See all GFR alpha 3/GFRA3 primary antibodies -
Description
Rabbit polyclonal to GFR alpha 3/GFRA3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab6100) -
General notes
Members of the glial cell line-derived neurotrophic factor(GDNF) family including GDNF and neurturin (NTN) play essential roles in the control of vertebrate neuron survival and differentiation. A new member of the GDNF family was recently identified and designated persephin. Physiological responses to these neurotrophic factors require two receptor subunits, the novel glycosylphosphadidylinositol linked protein GFRa and Ret receptor tyrosine kinase GFRb. Following the findings of GFRa-1 and -2, a novel receptor in GFRa family was identified very recently from human and mouse and designated GFRa-3. GFRa-3 binds persephin, thus, persephin, GFRa-3, and Ret PTK form a complex to transduce persephin signal and to mediate persephin function.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading...
-
Purity
DEAE-Chromatography -
Primary antibody notes
Members of the glial cell line-derived neurotrophic factor(GDNF) family including GDNF and neurturin (NTN) play essential roles in the control of vertebrate neuron survival and differentiation. A new member of the GDNF family was recently identified and designated persephin. Physiological responses to these neurotrophic factors require two receptor subunits, the novel glycosylphosphadidylinositol linked protein GFRa and Ret receptor tyrosine kinase GFRb. Following the findings of GFRa-1 and -2, a novel receptor in GFRa family was identified very recently from human and mouse and designated GFRa-3. GFRa-3 binds persephin, thus, persephin, GFRa-3, and Ret PTK form a complex to transduce persephin signal and to mediate persephin function. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Immunizing Peptide (Blocking)
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8028 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 10 µg/ml.
|
|
WB |
1/500 - 1/1000. Predicted molecular weight: 43 kDa.Can be blocked with GFR alpha 3/GFRA3 peptide (ab6100).
|
Notes |
---|
IHC-P
Use a concentration of 10 µg/ml. |
WB
1/500 - 1/1000. Predicted molecular weight: 43 kDa.Can be blocked with GFR alpha 3/GFRA3 peptide (ab6100). |
Target
-
Function
Receptor for the glial cell line-derived neurotrophic factor, ARTN (artemin). Mediates the artemin-induced autophosphorylation and activation of the RET receptor tyrosine kinase. -
Tissue specificity
Widely expressed in adult and fetus which exhibit a similar pattern. Essentially not expressed in the central nervous system, but highly expressed in several sensory and sympathetic ganglia of the peripheral nervous system. Moderate expression in many non-neuronal tissues, particularly those of the digestive and urogenital systems, but high expression in stomach and appendix. Several types of glandular tissues show low expression. Very low or no expression detected in the hematopoietic system. -
Sequence similarities
Belongs to the GDNFR family. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 14587 Mouse
- SwissProt: O35118 Mouse
- Unigene: 16520 Mouse
-
Alternative names
- GDNF family receptor alpha 3 antibody
- GDNF family receptor alpha-3 antibody
- GDNF receptor alpha-3 antibody
see all
Images
-
All lanes : Anti-GFR alpha 3/GFRA3 antibody (ab8028) at 1/500 dilution
Lane 1 : Crude cell membrane fractions of murine heart
Lane 2 : Crude cell membrane fractions of murine spleen
Lane 3 : Crude cell membrane fractions of murine kidney
Lane 4 : Crude cell membrane fractions of murine liver
Lane 5 : Crude cell membrane fractions of murine brain
Predicted band size: 43 kDa
Observed band size: 43 kDaWestern blot analysis of GFR alpha 3/GFRA3 in crude cell membrane fractions of
murine heart (H), spleen (S), kidney (K), liver (L), and brain (B), respectively, with ab8028 at 1/500 dilution. -
Immunohistochemistry (Formalin-fixed paraffin embedded sections) of mouse kidney tissue labeling GFR alpha 3/GFRA3 with Anti-GFR alpha 3/GFRA3 antibody (ab8028) at 5μg/ml.
-
ab8028 at 10µg/ml staining GFR alpha 3/GFRA3 in mouse heart tissue by IHC-P.
Protocols
Datasheets and documents
-
Datasheet download
References (9)
ab8028 has been referenced in 9 publications.
- Li TJ et al. Human splenic TER cells: A relevant prognostic factor acting via the artemin-GFRα3-ERK pathway in pancreatic ductal adenocarcinoma. Int J Cancer 148:1756-1767 (2021). PubMed: 33236361
- Rice FL et al. The evolution and multi-molecular properties of NF1 cutaneous neurofibromas originating from C-fiber sensory endings and terminal Schwann cells at normal sites of sensory terminations in the skin. PLoS One 14:e0216527 (2019). PubMed: 31107888
- Qi Q et al. Effect of moxibustion on the expression of GDNF and its receptor GFRa3 in the colon and spinal cord of rats with irritable bowel syndrome. Acupunct Med 37:244-251 (2019). PubMed: 31188015
- Han Y et al. Tumor-Induced Generation of Splenic Erythroblast-like Ter-Cells Promotes Tumor Progression. Cell 173:634-648.e12 (2018). PubMed: 29606356
- Ito Y et al. Identification of targets of tumor suppressor microRNA-34a using a reporter library system. Proc Natl Acad Sci U S A 114:3927-3932 (2017). PubMed: 28356515
- Omodaka K et al. Artemin augments survival and axon regeneration in axotomized retinal ganglion cells. J Neurosci Res 92:1637-46 (2014). PubMed: 25044131
- Kawamura K et al. Promotion of human early embryonic development and blastocyst outgrowth in vitro using autocrine/paracrine growth factors. PLoS One 7:e49328 (2012). IHC - Wholemount ; Human . PubMed: 23152897
- Ceyhan GO et al. The neurotrophic factor artemin promotes pancreatic cancer invasion. Ann Surg 244:274-81 (2006). WB, IHC-P ; Human . PubMed: 16858191
- Serra MP et al. Ret, GFRalpha-1, GFRalpha-2 and GFRalpha-3 receptors in the human hippocampus and fascia dentata. Int J Dev Neurosci 23:425-38 (2005). PubMed: 16002253