Product nameAnti-GGA1 antibody
See all GGA1 primary antibodies
DescriptionRabbit polyclonal to GGA1
SpecificityThe immunizing seqeunce is present only in isoform 1 of this protein, therefore cross-reactivity with splice variants is not expected.
Tested applicationsSuitable for: ICC/IF, WB, ELISAmore details
Species reactivityReacts with: Human
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab10551 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent dilution.|
|WB||1/500 - 1/3000. Predicted molecular weight: 70 kDa.|
|ELISA||1/10000 - 1/50000.|
FunctionPlays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the GGA protein family.
Contains 1 GAE domain.
Contains 1 GAT domain.
Contains 1 VHS domain.
DomainThe VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (AC-LL motif).
The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis.
The unstructured hinge region contains clathrin-binding but no autoinhibitory (AC-LL) motifs.
The GAE domain binds accessory proteins regulating GGAs function.
modificationsPhosphorylated by CK2 and dephosphorylated by PP2A. Phosphorylation of GGA1 allows the internal AC-LL motif to bind the VHS domain and to inhibit the recognition of cargo signals. Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationGolgi apparatus > trans-Golgi network membrane. Endosome membrane.
- Information by UniProt
- 4930406E12Rik antibody
- ADP ribosylation factor binding protein 1 antibody
- ADP ribosylation factor binding protein GGA1 antibody
Anti-GGA1 antibody (ab10551) at 1/1350 dilution + Lysate of HEK293 cells overexpressing YFP-GGA1 fusion protein at 35 µg/ml
Goat-anti Rabbit IgG
Performed under reducing conditions.
Predicted band size: 70 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
The lysate prepared from HEK293 cells overexpressing YFP-GGA1 fusion protein. This accounts for the observed band of 100 kDa.
This product has been referenced in:
- Lefrançois S & McCormick PJ The Arf GEF GBF1 is required for GGA recruitment to Golgi membranes. Traffic 8:1440-51 (2007). ICC/IF ; African green monkey . Read more (PubMed: 17666033) »
- Wahle T et al. GGA1 is expressed in the human brain and affects the generation of amyloid beta-peptide. J Neurosci 26:12838-46 (2006). WB ; Human . Read more (PubMed: 17151287) »