Key features and details
- Rabbit polyclonal to GGA1
- Suitable for: ICC/IF, WB, ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GGA1 antibody
See all GGA1 primary antibodies
DescriptionRabbit polyclonal to GGA1
SpecificityThe immunizing seqeunce is present only in isoform 1 of this protein, therefore cross-reactivity with splice variants is not expected.
Tested applicationsSuitable for: ICC/IF, WB, ELISAmore details
Species reactivityReacts with: Human
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab10551 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent dilution.|
|WB||1/500 - 1/3000. Predicted molecular weight: 70 kDa.|
|ELISA||1/10000 - 1/50000.|
FunctionPlays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the GGA protein family.
Contains 1 GAE domain.
Contains 1 GAT domain.
Contains 1 VHS domain.
DomainThe VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (AC-LL motif).
The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis.
The unstructured hinge region contains clathrin-binding but no autoinhibitory (AC-LL) motifs.
The GAE domain binds accessory proteins regulating GGAs function.
modificationsPhosphorylated by CK2 and dephosphorylated by PP2A. Phosphorylation of GGA1 allows the internal AC-LL motif to bind the VHS domain and to inhibit the recognition of cargo signals. Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationGolgi apparatus > trans-Golgi network membrane. Endosome membrane.
- Information by UniProt
- 4930406E12Rik antibody
- ADP ribosylation factor binding protein 1 antibody
- ADP ribosylation factor binding protein GGA1 antibody
Anti-GGA1 antibody (ab10551) at 1/1350 dilution + Lysate of HEK293 cells overexpressing YFP-GGA1 fusion protein at 35 µg/ml
Goat-anti Rabbit IgG
Performed under reducing conditions.
Predicted band size: 70 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
The lysate prepared from HEK293 cells overexpressing YFP-GGA1 fusion protein. This accounts for the observed band of 100 kDa.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab10551 has been referenced in 2 publications.
- Lefrançois S & McCormick PJ The Arf GEF GBF1 is required for GGA recruitment to Golgi membranes. Traffic 8:1440-51 (2007). ICC/IF ; African green monkey . PubMed: 17666033
- Wahle T et al. GGA1 is expressed in the human brain and affects the generation of amyloid beta-peptide. J Neurosci 26:12838-46 (2006). WB ; Human . PubMed: 17151287