Key features and details
- Rabbit polyclonal to GGA1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GGA1 antibody
See all GGA1 primary antibodies
DescriptionRabbit polyclonal to GGA1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
Recombinant fragment, corresponding to a region within amino acids 46-307 of Human GGA1.
- NT2D1, PC-3, U87-MG and SK-N-SH whole cell lysates; Human gastric carcinoma tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78% PBS, 1% BSA, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab153799 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 70 kDa.|
|IHC-P||1/100 - 1/1000.|
FunctionPlays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the GGA protein family.
Contains 1 GAE domain.
Contains 1 GAT domain.
Contains 1 VHS domain.
DomainThe VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (AC-LL motif).
The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis.
The unstructured hinge region contains clathrin-binding but no autoinhibitory (AC-LL) motifs.
The GAE domain binds accessory proteins regulating GGAs function.
modificationsPhosphorylated by CK2 and dephosphorylated by PP2A. Phosphorylation of GGA1 allows the internal AC-LL motif to bind the VHS domain and to inhibit the recognition of cargo signals. Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationGolgi apparatus > trans-Golgi network membrane. Endosome membrane.
- Information by UniProt
- 4930406E12Rik antibody
- ADP ribosylation factor binding protein 1 antibody
- ADP ribosylation factor binding protein GGA1 antibody
All lanes : Anti-GGA1 antibody (ab153799) at 1/1000 dilution
Lane 1 : NT2D1 whole cell
Lane 2 : PC-3 whole cell
Lane 3 : U87-MG whole cell
Lane 4 : SK-N-SH whole cell
Lysates/proteins at 30 µg per lane.
Predicted band size: 70 kDa
7.5% SDS PAGE
Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling GGA1 with ab153799 at 1/500 dilution.
ab153799 has not yet been referenced specifically in any publications.