Overview

  • Product name

  • Description

    Rabbit polyclonal to GGA1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human GGA1 aa 300-350. The exact sequence is proprietary.
    Database link: Q9UJY5

  • Positive control

    • IP: HEK-293T whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    Antibody was affinity purified using an epitope specific to GGA1 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab241124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif.
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Belongs to the GGA protein family.
    Contains 1 GAE domain.
    Contains 1 GAT domain.
    Contains 1 VHS domain.
  • Domain

    The VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (AC-LL motif).
    The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis.
    The unstructured hinge region contains clathrin-binding but no autoinhibitory (AC-LL) motifs.
    The GAE domain binds accessory proteins regulating GGAs function.
  • Post-translational
    modifications

    Phosphorylated by CK2 and dephosphorylated by PP2A. Phosphorylation of GGA1 allows the internal AC-LL motif to bind the VHS domain and to inhibit the recognition of cargo signals. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Ubiquitinated.
  • Cellular localization

    Golgi apparatus > trans-Golgi network membrane. Endosome membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 4930406E12Rik antibody
    • ADP ribosylation factor binding protein 1 antibody
    • ADP ribosylation factor binding protein GGA1 antibody
    • ADP-ribosylation factor-binding protein GGA1 antibody
    • ARF binding protein 1 antibody
    • ARF-binding protein 1 antibody
    • Gamma adaptin related protein 1 antibody
    • gamma ear-containing antibody
    • Gamma-adaptin-related protein 1 antibody
    • GGA 1 antibody
    • GGA1 antibody
    • GGA1 protein antibody
    • GGA1_HUMAN antibody
    • Golgi associated gamma adaptin ear containing ARF antibody
    • Golgi associated gamma adaptin ear containing ARF binding protein 1 antibody
    • Golgi localized gamma ear containing ARF binding protein 1 antibody
    • Golgi-localized antibody
    • OTTHUMP00000028975 antibody
    • OTTHUMP00000042200 antibody
    see all

Images

  • GGA1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241124 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241124 at 0.4 µg/ml.

    Lane 1: ab241124 IP in HEK-293T whole cell lysate.
    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection: Chemiluminescence with exposure time of 10 seconds.

    Lysates prepared in NETN buffer.

References

ab241124 has not yet been referenced specifically in any publications.

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