Overview

  • Product name
    Anti-GGA1 antibody [EPR3014(2)]
    See all GGA1 primary antibodies
  • Description
    Rabbit monoclonal [EPR3014(2)] to GGA1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human GGA1 aa 100-200 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9UJY5

  • Positive control
    • HeLa, Jurkat, 293T and K562 cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR3014(2)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 70 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function
      Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif.
    • Tissue specificity
      Ubiquitously expressed.
    • Sequence similarities
      Belongs to the GGA protein family.
      Contains 1 GAE domain.
      Contains 1 GAT domain.
      Contains 1 VHS domain.
    • Domain
      The VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (AC-LL motif).
      The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis.
      The unstructured hinge region contains clathrin-binding but no autoinhibitory (AC-LL) motifs.
      The GAE domain binds accessory proteins regulating GGAs function.
    • Post-translational
      modifications
      Phosphorylated by CK2 and dephosphorylated by PP2A. Phosphorylation of GGA1 allows the internal AC-LL motif to bind the VHS domain and to inhibit the recognition of cargo signals. Phosphorylated upon DNA damage, probably by ATM or ATR.
      Ubiquitinated.
    • Cellular localization
      Golgi apparatus > trans-Golgi network membrane. Endosome membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 4930406E12Rik antibody
      • ADP ribosylation factor binding protein 1 antibody
      • ADP ribosylation factor binding protein GGA1 antibody
      • ADP-ribosylation factor-binding protein GGA1 antibody
      • ARF binding protein 1 antibody
      • ARF-binding protein 1 antibody
      • Gamma adaptin related protein 1 antibody
      • gamma ear-containing antibody
      • Gamma-adaptin-related protein 1 antibody
      • GGA 1 antibody
      • GGA1 antibody
      • GGA1 protein antibody
      • GGA1_HUMAN antibody
      • Golgi associated gamma adaptin ear containing ARF antibody
      • Golgi associated gamma adaptin ear containing ARF binding protein 1 antibody
      • Golgi localized gamma ear containing ARF binding protein 1 antibody
      • Golgi-localized antibody
      • OTTHUMP00000028975 antibody
      • OTTHUMP00000042200 antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: GGA1 knockout HAP1 cell lysate (20 µg)
      Lane 3: Jurkat cell lysate (20 µg)
      Lane 4: HeLa cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab170956 observed at 75 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab170956 was shown to recognize GGA1 when GGA1 knockout samples were used, along with additional cross-reactive bands. Wild-type and GGA1 knockout samples were subjected to SDS-PAGE. ab170956 and ab8245 (loading control to GAPDH) were diluted 2 µg/ml and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Western blot analysis on Immunoprecipitation pellet from Jurkat cells showing GGA1, using ab170956 at 1/10 dilution for IP.

    • All lanes : Anti-GGA1 antibody [EPR3014(2)] (ab170956) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 70 kDa

    References

    This product has been referenced in:
    • Toh WH  et al. GGA1 regulates signal-dependent sorting of BACE1 to recycling endosomes, which moderates Aß production. Mol Biol Cell 29:191-208 (2018). Read more (PubMed: 29142073) »
    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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