Anti-GGA2 antibody (ab10552)

Submit a review Q&A (1)References (2)

Overview

  • Product name
    Anti-GGA2 antibody
    See all GGA2 primary antibodies
  • Description
    Rabbit polyclonal to GGA2
  • Host species
    Rabbit
  • Specificity
    This antibody recognises two bands by Western at 75 and 37kD. This corresponds to results seen in Hela extracts in Dell'Angelica et al. 2000. The lower 37kD band is expected to correspond to the isoform described in NP_619581.
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide:

    LSMYRRPGQAPPDQEAL

    conjugated to KLH, corresponding to amino acids 249-265 of Human GGA2.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
WB 1/500. Detects a band of approximately 75, 37 kDa (predicted molecular weight: 67 kDa).

Target

  • Function
    Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif.
  • Tissue specificity
    Ubiquitously expressed.
  • Sequence similarities
    Belongs to the GGA protein family.
    Contains 1 GAE domain.
    Contains 1 GAT domain.
    Contains 1 VHS domain.
  • Domain
    The VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (AC-LL motif).
    The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis.
    The unstructured hinge region contains clathrin-binding but no autoinhibitory (AC-LL) motifs.
    The GAE domain binds accessory proteins regulating GGAs function.
  • Post-translational
    modifications
    Ubiquitinated.
  • Cellular localization
    Golgi apparatus > trans-Golgi network membrane. Endosome membrane.
  • Information by UniProt
  • Database links
    • Alternative names
      • 1200007E24Rik antibody
      • ADP ribosylation factor binding protein 2 antibody
      • ADP ribosylation factor binding protein GGA 2 antibody
      • ADP ribosylation factor binding protein GGA2 antibody
      • ADP-ribosylation factor-binding protein GGA2 antibody
      • ARF-binding protein 2 antibody
      • FLJ20966 antibody
      • Gamma adaptin related protein 2 antibody
      • gamma ear-containing antibody
      • Gamma-adaptin-related protein 2 antibody
      • GGA 2 antibody
      • Gga2 antibody
      • GGA2_HUMAN antibody
      • Golgi associated gamma adaptin ear containing ARF binding protein 2 antibody
      • Golgi localized gamma ear containing ARF binding protein 2 antibody
      • Golgi-localized antibody
      • KIAA1080 antibody
      • Vear antibody
      • VHS domain and ear domain containing protein antibody
      • VHS domain and ear domain of gamma adaptin antibody
      • VHS domain and ear domain of gamma-adaptin antibody
      see all

    Images

    • Western blot - Anti-GGA2 antibody (ab10552)
      Western blot - Anti-GGA2 antibody (ab10552)

      Western blot using ab10552 at 1/500. Lysates at 5µg per lane. Secondary antibody anti-rabbit HRP ab6721 at 1/5000.
           
      Lane 1: Hela nuclear
      Lane 2: Hela
      Lane 3: A431
      Lane 4: Jurkat
      Lane 5: 293

      Western blot using ab10552 at 1/500. Lysates at 5µg per lane. Secondary ab6721 at 1/5000. Lane 1: Hela nuclear Lane 2: Hela Lane 3: A431 Lane 4: Jurkat Lane 5: 293

    References

    This product has been referenced in:
    • Herskowitz JH  et al. GGA1-mediated endocytic traffic of LR11/SorLA alters APP intracellular distribution and amyloid-ß production. Mol Biol Cell 23:2645-57 (2012). WB, ICC/IF ; Human . Read more (PubMed: 22621900) »
    • Lefrançois S & McCormick PJ The Arf GEF GBF1 is required for GGA recruitment to Golgi membranes. Traffic 8:1440-51 (2007). ICC/IF ; African green monkey . Read more (PubMed: 17666033) »
    See all 2 Publications for this product

    Publishing research using ab10552? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    Hi, I'm looking for a good GGA2 antibody for ICC in rabbit cultured cells or IHC in mouse tissue. I saw the reference on your page to a reasonably good ICC picture for Cos7 cells and thought it might be worthwhile to try in our cells- but was wondering: what is the sequence homology between the human peptide sequence used for this antibody and the mouse GGA2? Do you think there would be a good chance for positive results? Thanks.

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    Answer

    Thank you for your enquiry. As far as we are aware, cross reactivity with mouse has not yet been tested for use with ab10552. All species cross-reactivity information is stated on Abcam product datasheets, and these are updated as soon as any new information is brought to our attention. However, I have run a BLAST search (results below) using the immunogen sequence and found that there is 82% homolgy with mouse. We would normally expect a high chance of positive results if the homology was more than 90%. However, this does not mean that the antibody will not work, just that we have not tested it and therefore, can not guarantee that you will get good results. GENE ID: 74105 Gga2 | golgi associated, gamma adaptin ear containing, ARF binding protein 2 [Mus musculus] (Over 10 PubMed links) Score = 46.4 bits (102), Expect = 6e-06 Identities = 14/17 (82%), Positives = 15/17 (88%), Gaps = 0/17 (0%) Query 1 LSMYRRPGQAPPDQEAL 17 LSMYRRPG A PDQ+AL Sbjct 2 LSMYRRPGHALPDQQAL 18 If you decide to go ahead and purchase this product, we encourage you to submit an Abreview via the online product datasheet. We always encourage customers to send their results back to us, whether positive or negative, and we make all product information available to researchers. To find out more about our Abreview system, please see the following URL: https://www.abcam.com/abreviews I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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