Anti-GGA2 antibody (ab10552)

Submit a review Q&A (1)References (3)

Key features and details

  • Rabbit polyclonal to GGA2
  • Suitable for: ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-GGA2 antibody
    See all GGA2 primary antibodies

  • Description

    Rabbit polyclonal to GGA2

  • Host species

    Rabbit

  • Specificity

    This antibody recognises two bands by Western at 75 and 37kD. This corresponds to results seen in Hela extracts in Dell'Angelica et al. 2000. The lower 37kD band is expected to correspond to the isoform described in NP_619581.

  • Tested applications

    Suitable for: ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse

  • Immunogen

    Synthetic peptide:

    LSMYRRPGQAPPDQEAL

    conjugated to KLH, corresponding to amino acids 249-265 of Human GGA2.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
WB 1/500. Detects a band of approximately 75, 37 kDa (predicted molecular weight: 67 kDa).

Target

  • Function

    Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif.

  • Tissue specificity

    Ubiquitously expressed.

  • Sequence similarities

    Belongs to the GGA protein family.
    Contains 1 GAE domain.
    Contains 1 GAT domain.
    Contains 1 VHS domain.

  • Domain

    The VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (AC-LL motif).
    The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis.
    The unstructured hinge region contains clathrin-binding but no autoinhibitory (AC-LL) motifs.
    The GAE domain binds accessory proteins regulating GGAs function.

  • Post-translational
    modifications

    Ubiquitinated.

  • Cellular localization

    Golgi apparatus > trans-Golgi network membrane. Endosome membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • 1200007E24Rik antibody
      • ADP ribosylation factor binding protein 2 antibody
      • ADP ribosylation factor binding protein GGA 2 antibody
      • ADP ribosylation factor binding protein GGA2 antibody
      • ADP-ribosylation factor-binding protein GGA2 antibody
      • ARF-binding protein 2 antibody
      • FLJ20966 antibody
      • Gamma adaptin related protein 2 antibody
      • gamma ear-containing antibody
      • Gamma-adaptin-related protein 2 antibody
      • GGA 2 antibody
      • Gga2 antibody
      • GGA2_HUMAN antibody
      • Golgi associated gamma adaptin ear containing ARF binding protein 2 antibody
      • Golgi localized gamma ear containing ARF binding protein 2 antibody
      • Golgi-localized antibody
      • KIAA1080 antibody
      • Vear antibody
      • VHS domain and ear domain containing protein antibody
      • VHS domain and ear domain of gamma adaptin antibody
      • VHS domain and ear domain of gamma-adaptin antibody
      see all

    Images

    • Western blot - Anti-GGA2 antibody (ab10552)
      Western blot - Anti-GGA2 antibody (ab10552)

      Western blot using ab10552 at 1/500. Lysates at 5µg per lane. Secondary antibody anti-rabbit HRP ab6721 at 1/5000.
           
      Lane 1: Hela nuclear
      Lane 2: Hela
      Lane 3: A431
      Lane 4: Jurkat
      Lane 5: 293

      Western blot using ab10552 at 1/500. Lysates at 5µg per lane. Secondary ab6721 at 1/5000. Lane 1: Hela nuclear Lane 2: Hela Lane 3: A431 Lane 4: Jurkat Lane 5: 293

    References

    Publishing research using ab10552? Please let us know so that we can cite the reference in this datasheet.

    ab10552 has been referenced in 3 publications.

    • Duffney LJ  et al. Epigenetics and autism spectrum disorder: A report of an autism case with mutation in H1 linker histone HIST1H1E and literature review. Am J Med Genet B Neuropsychiatr Genet 177:426-433 (2018). PubMed: 29704315
    • Herskowitz JH  et al. GGA1-mediated endocytic traffic of LR11/SorLA alters APP intracellular distribution and amyloid-ß production. Mol Biol Cell 23:2645-57 (2012). WB, ICC/IF ; Human . PubMed: 22621900
    • Lefrançois S & McCormick PJ The Arf GEF GBF1 is required for GGA recruitment to Golgi membranes. Traffic 8:1440-51 (2007). ICC/IF ; African green monkey . PubMed: 17666033

    Customer reviews and Q&As

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    Question

    Hi, I'm looking for a good GGA2 antibody for ICC in rabbit cultured cells or IHC in mouse tissue. I saw the reference on your page to a reasonably good ICC picture for Cos7 cells and thought it might be worthwhile to try in our cells- but was wondering: what is the sequence homology between the human peptide sequence used for this antibody and the mouse GGA2? Do you think there would be a good chance for positive results? Thanks.

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    Answer

    Thank you for your enquiry. As far as we are aware, cross reactivity with mouse has not yet been tested for use with ab10552. All species cross-reactivity information is stated on Abcam product datasheets, and these are updated as soon as any new information is brought to our attention. However, I have run a BLAST search (results below) using the immunogen sequence and found that there is 82% homolgy with mouse. We would normally expect a high chance of positive results if the homology was more than 90%. However, this does not mean that the antibody will not work, just that we have not tested it and therefore, can not guarantee that you will get good results. GENE ID: 74105 Gga2 | golgi associated, gamma adaptin ear containing, ARF binding protein 2 [Mus musculus] (Over 10 PubMed links) Score = 46.4 bits (102), Expect = 6e-06 Identities = 14/17 (82%), Positives = 15/17 (88%), Gaps = 0/17 (0%) Query 1 LSMYRRPGQAPPDQEAL 17 LSMYRRPG A PDQ+AL Sbjct 2 LSMYRRPGHALPDQQAL 18 If you decide to go ahead and purchase this product, we encourage you to submit an Abreview via the online product datasheet. We always encourage customers to send their results back to us, whether positive or negative, and we make all product information available to researchers. To find out more about our Abreview system, please see the following URL: https://www.abcam.com/abreviews I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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