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  1. Link

    gga2-antibody-ab153928.pdf

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Signal Transduction Protein Trafficking Organelle Proteins
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Anti-GGA2 antibody (ab153928)

  • Datasheet
  • SDS
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Abpromise

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Western blot - Anti-GGA2 antibody (ab153928)
  • Immunocytochemistry/ Immunofluorescence - Anti-GGA2 antibody (ab153928)

Key features and details

  • Rabbit polyclonal to GGA2
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-GGA2 antibody
    See all GGA2 primary antibodies
  • Description

    Rabbit polyclonal to GGA2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 20-280 of Human GGA2 (UniProt: Q9UJY4).

  • Positive control

    • HepG2, MOLT4 and Raji whole cell lysates; HepG2 cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Organelle Proteins

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Raji whole cell lysate (ab30124)
    • MOLT4 whole cell lysate (ab7912)

Applications

Our Abpromise guarantee covers the use of ab153928 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 67 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif.
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Belongs to the GGA protein family.
    Contains 1 GAE domain.
    Contains 1 GAT domain.
    Contains 1 VHS domain.
  • Domain

    The VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (AC-LL motif).
    The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis.
    The unstructured hinge region contains clathrin-binding but no autoinhibitory (AC-LL) motifs.
    The GAE domain binds accessory proteins regulating GGAs function.
  • Post-translational
    modifications

    Ubiquitinated.
  • Cellular localization

    Golgi apparatus > trans-Golgi network membrane. Endosome membrane.
  • Target information above from: UniProt accession Q9UJY4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 23062 Human
    • Entrez Gene: 74105 Mouse
    • Entrez Gene: 293455 Rat
    • Omim: 606005 Human
    • SwissProt: Q9UJY4 Human
    • SwissProt: Q6P5E6 Mouse
    • Unigene: 460336 Human
    • Unigene: 29619 Mouse
    • Unigene: 18248 Rat
    see all
  • Alternative names

    • 1200007E24Rik antibody
    • ADP ribosylation factor binding protein 2 antibody
    • ADP ribosylation factor binding protein GGA 2 antibody
    • ADP ribosylation factor binding protein GGA2 antibody
    • ADP-ribosylation factor-binding protein GGA2 antibody
    • ARF-binding protein 2 antibody
    • FLJ20966 antibody
    • Gamma adaptin related protein 2 antibody
    • gamma ear-containing antibody
    • Gamma-adaptin-related protein 2 antibody
    • GGA 2 antibody
    • Gga2 antibody
    • GGA2_HUMAN antibody
    • Golgi associated gamma adaptin ear containing ARF binding protein 2 antibody
    • Golgi localized gamma ear containing ARF binding protein 2 antibody
    • Golgi-localized antibody
    • KIAA1080 antibody
    • Vear antibody
    • VHS domain and ear domain containing protein antibody
    • VHS domain and ear domain of gamma adaptin antibody
    • VHS domain and ear domain of gamma-adaptin antibody
    see all

Images

  • Western blot - Anti-GGA2 antibody (ab153928)
    Western blot - Anti-GGA2 antibody (ab153928)
    Anti-GGA2 antibody (ab153928) at 1/10000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size: 67 kDa



    7.5% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-GGA2 antibody (ab153928)
    Immunocytochemistry/ Immunofluorescence - Anti-GGA2 antibody (ab153928)
    Immunofluorescent analysis of methanol-fixed HepG2 cells, labeling GGA2 with ab153928 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab153928? Please let us know so that we can cite the reference in this datasheet.

    ab153928 has not yet been referenced specifically in any publications.

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