Anti-GGA2 antibody (ab153928)
Key features and details
- Rabbit polyclonal to GGA2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GGA2 antibody
See all GGA2 primary antibodies -
Description
Rabbit polyclonal to GGA2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 20-280 of Human GGA2 (UniProt: Q9UJY4).
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Positive control
- HepG2, MOLT4 and Raji whole cell lysates; HepG2 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab153928 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000 - 1/20000. Predicted molecular weight: 67 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the GGA protein family.
Contains 1 GAE domain.
Contains 1 GAT domain.
Contains 1 VHS domain. -
Domain
The VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (AC-LL motif).
The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis.
The unstructured hinge region contains clathrin-binding but no autoinhibitory (AC-LL) motifs.
The GAE domain binds accessory proteins regulating GGAs function. -
Post-translational
modificationsUbiquitinated. -
Cellular localization
Golgi apparatus > trans-Golgi network membrane. Endosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 23062 Human
- Entrez Gene: 74105 Mouse
- Entrez Gene: 293455 Rat
- Omim: 606005 Human
- SwissProt: Q9UJY4 Human
- SwissProt: Q6P5E6 Mouse
- Unigene: 460336 Human
- Unigene: 29619 Mouse
see all -
Alternative names
- 1200007E24Rik antibody
- ADP ribosylation factor binding protein 2 antibody
- ADP ribosylation factor binding protein GGA 2 antibody
see all
Images
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Western blot - Anti-GGA2 antibody (ab153928)Anti-GGA2 antibody (ab153928) at 1/10000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 67 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-GGA2 antibody (ab153928)Immunofluorescent analysis of methanol-fixed HepG2 cells, labeling GGA2 with ab153928 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
Protocols
References (0)
ab153928 has not yet been referenced specifically in any publications.
