Anti-GGA2 antibody (ab153928)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-GGA2 antibody
See all GGA2 primary antibodies -
Description
Rabbit polyclonal to GGA2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 20-280 of Human GGA2 (UniProt: Q9UJY4).
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Positive control
- HepG2, MOLT4 and Raji whole cell lysates; HepG2 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab153928 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000 - 1/20000. Predicted molecular weight: 67 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the GGA protein family.
Contains 1 GAE domain.
Contains 1 GAT domain.
Contains 1 VHS domain. -
Domain
The VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (AC-LL motif).
The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis.
The unstructured hinge region contains clathrin-binding but no autoinhibitory (AC-LL) motifs.
The GAE domain binds accessory proteins regulating GGAs function. -
Post-translational
modificationsUbiquitinated. -
Cellular localization
Golgi apparatus > trans-Golgi network membrane. Endosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 23062 Human
- Entrez Gene: 74105 Mouse
- Entrez Gene: 293455 Rat
- Omim: 606005 Human
- SwissProt: Q9UJY4 Human
- SwissProt: Q6P5E6 Mouse
- Unigene: 460336 Human
- Unigene: 29619 Mouse
see all -
Alternative names
- 1200007E24Rik antibody
- ADP ribosylation factor binding protein 2 antibody
- ADP ribosylation factor binding protein GGA 2 antibody
see all
Images
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Western blot - Anti-GGA2 antibody (ab153928)Anti-GGA2 antibody (ab153928) at 1/10000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 67 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-GGA2 antibody (ab153928)Immunofluorescent analysis of methanol-fixed HepG2 cells, labeling GGA2 with ab153928 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
References
ab153928 has not yet been referenced specifically in any publications.
