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- Specific References
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Overview
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Product nameAnti-GGA2 antibody
See all GGA2 primary antibodies -
DescriptionRabbit polyclonal to GGA2
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IFmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 20-280 of Human GGA2 (UniProt: Q9UJY4).
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Positive control
- HepG2, MOLT4 and Raji whole cell lysates; HepG2 cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab153928 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000 - 1/20000. Predicted molecular weight: 67 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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FunctionPlays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif.
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Tissue specificityUbiquitously expressed.
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Sequence similaritiesBelongs to the GGA protein family.
Contains 1 GAE domain.
Contains 1 GAT domain.
Contains 1 VHS domain. -
DomainThe VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (AC-LL motif).
The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis.
The unstructured hinge region contains clathrin-binding but no autoinhibitory (AC-LL) motifs.
The GAE domain binds accessory proteins regulating GGAs function. -
Post-translational
modificationsUbiquitinated. -
Cellular localizationGolgi apparatus > trans-Golgi network membrane. Endosome membrane.
- Information by UniProt
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Database links
- Entrez Gene: 23062 Human
- Entrez Gene: 74105 Mouse
- Entrez Gene: 293455 Rat
- Omim: 606005 Human
- SwissProt: Q9UJY4 Human
- SwissProt: Q6P5E6 Mouse
- Unigene: 460336 Human
- Unigene: 29619 Mouse
see all -
Alternative names
- 1200007E24Rik antibody
- ADP ribosylation factor binding protein 2 antibody
- ADP ribosylation factor binding protein GGA 2 antibody
see all
Images
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Western blot - Anti-GGA2 antibody (ab153928)Anti-GGA2 antibody (ab153928) at 1/10000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 67 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-GGA2 antibody (ab153928)Immunofluorescent analysis of methanol-fixed HepG2 cells, labeling GGA2 with ab153928 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
References
ab153928 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"