Anti-GGA3 antibody (ab10553)
- Datasheet
- References (3)
- Protocols
Overview
-
Product name
Anti-GGA3 antibody
See all GGA3 primary antibodies -
Description
Rabbit polyclonal to GGA3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC (PFA fixed)more details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide:
ADPAPNVPPKESAGNS
conjugated to KLH, corresponding to amino acids 400-415 of Human GGA3. -
General notes
Expiration date is 1 year from date of opening.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
Our Abpromise guarantee covers the use of ab10553 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 78 kDa. | |
| IHC (PFA fixed) | 1/40. PubMed: 17116753 |
Target
-
Function
Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the GGA protein family.
Contains 1 GAE domain.
Contains 1 GAT domain.
Contains 1 VHS domain. -
Domain
The VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (AC-LL motif).
The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis.
The unstructured hinge region contains clathrin-binding and an autoinhibitory (AC-LL) motifs.
The GAE domain binds accessory proteins regulating GGAs function. -
Post-translational
modificationsPhosphorylated by CK2 and dephosphorylated by PP2A (By similarity). Phosphorylation of GGA3 allows the internal AC-LL motif to bind the VHS domain and to inhibit the recognition of cargo signals.
Ubiquitinated. -
Cellular localization
Golgi apparatus > trans-Golgi network membrane. Endosome membrane. - Information by UniProt
-
Database links
- Entrez Gene: 23163 Human
- Omim: 606006 Human
- SwissProt: Q9NZ52 Human
- Unigene: 87726 Human
-
Alternative names
- ADP ribosylation factor binding protein 3 antibody
- ADP ribosylation factor binding protein GGA 3 antibody
- ADP ribosylation factor binding protein GGA3 antibody
see all
Images
-
Western blot - Anti-GGA3 antibody (ab10553)Anti-GGA3 antibody (ab10553) at 1/600 dilution + HEK293 lysate from cells transfected with GFP-GGA3 fusion protein at 35 µg
Secondary
IRDye 800 conjugated Goat-anti-Rabbit IgG [H&L] at 1/10000 dilution
Predicted band size: 78 kDa
A 110kDa band (see arrow) corresponding to the GFP-GGA3 fusion protein is detected by ab10553.
Datasheets and documents
References
This product has been referenced in:
- Wei R et al. Antagonistic Glucagon Receptor Antibody Promotes a-Cell Proliferation and Increases ß-Cell Mass in Diabetic Mice. iScience 16:326-339 (2019). Read more (PubMed: 31203188) »
- Koike MA et al. Oligemic hypoperfusion differentially affects tau and amyloid-{beta}. Am J Pathol 177:300-10 (2010). WB ; Mouse . Read more (PubMed: 20472896) »
