Anti-GGA3 antibody (ab10553)

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Western blot - Anti-GGA3 antibody (ab10553)

    Key features and details

    • Rabbit polyclonal to GGA3
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-GGA3 antibody
      See all GGA3 primary antibodies

    • Description

      Rabbit polyclonal to GGA3

    • Host species

      Rabbit

    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data

    • Immunogen

      Synthetic peptide:

      ADPAPNVPPKESAGNS

      conjugated to KLH, corresponding to amino acids 400-415 of Human GGA3.
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • General notes

      Expiration date is 1 year from date of opening.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • Storage buffer

      Preservative: 0.01% Sodium azide
      Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab10553 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    Application Abreviews Notes
    WB
    1/500 - 1/3000. Predicted molecular weight: 78 kDa.
    Notes
    WB
    1/500 - 1/3000. Predicted molecular weight: 78 kDa.

    Target

    • Function

      Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif.

    • Tissue specificity

      Ubiquitously expressed.

    • Sequence similarities

      Belongs to the GGA protein family.
      Contains 1 GAE domain.
      Contains 1 GAT domain.
      Contains 1 VHS domain.

    • Domain

      The VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (AC-LL motif).
      The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis.
      The unstructured hinge region contains clathrin-binding and an autoinhibitory (AC-LL) motifs.
      The GAE domain binds accessory proteins regulating GGAs function.

    • Post-translational
      modifications

      Phosphorylated by CK2 and dephosphorylated by PP2A (By similarity). Phosphorylation of GGA3 allows the internal AC-LL motif to bind the VHS domain and to inhibit the recognition of cargo signals.
      Ubiquitinated.

    • Cellular localization

      Golgi apparatus > trans-Golgi network membrane. Endosome membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • ADP ribosylation factor binding protein 3 antibody
        • ADP ribosylation factor binding protein GGA 3 antibody
        • ADP ribosylation factor binding protein GGA3 antibody
        • ADP-ribosylation factor-binding protein GGA3 antibody
        • ARF binding protein GGA 3 antibody
        • ARF binding protein GGA3 antibody
        • ARF-binding protein 3 antibody
        • gamma ear-containing antibody
        • GGA 3 antibody
        • GGA3 antibody
        • GGA3_HUMAN antibody
        • Golgi associated gamma adaptin ear containing ARF binding protein 3 antibody
        • Golgi localized gamma ear containing ARF binding protein 3 antibody
        • Golgi-localized antibody
        • KIAA0154 antibody
        see all

      Images

      • Western blot - Anti-GGA3 antibody (ab10553)
        Western blot - Anti-GGA3 antibody (ab10553)
        Anti-GGA3 antibody (ab10553) at 1/600 dilution + HEK293 lysate from cells transfected with GFP-GGA3 fusion protein at 35 µg

        Secondary
        IRDye 800 conjugated Goat-anti-Rabbit IgG [H&L] at 1/10000 dilution

        Predicted band size: 78 kDa



        A 110kDa band (see arrow) corresponding to the GFP-GGA3 fusion protein is detected by ab10553.

      References (3)

      Publishing research using ab10553? Please let us know so that we can cite the reference in this datasheet.

      ab10553 has been referenced in 3 publications.

      • Wei R  et al. Antagonistic Glucagon Receptor Antibody Promotes a-Cell Proliferation and Increases ß-Cell Mass in Diabetic Mice. iScience 16:326-339 (2019). PubMed: 31203188
      • Koike MA  et al. Oligemic hypoperfusion differentially affects tau and amyloid-{beta}. Am J Pathol 177:300-10 (2010). WB ; Mouse . PubMed: 20472896
      • Pak Y  et al. Transport of LAPTM5 to lysosomes requires association with the ubiquitin ligase Nedd4, but not LAPTM5 ubiquitination. J Cell Biol 175:631-45 (2006). Electron Microscopy ; Human . PubMed: 17116753

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