Anti-GGA3 antibody (ab10553)
- Datasheet
- References (2)
- Protocols
Overview
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Product nameAnti-GGA3 antibody
See all GGA3 primary antibodies -
DescriptionRabbit polyclonal to GGA3
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC (PFA fixed)more details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide:
ADPAPNVPPKESAGNS
conjugated to KLH, corresponding to amino acids 400-415 of Human GGA3. -
General notesExpiration date is 1 year from date of opening.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab10553 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 78 kDa. | |
| IHC (PFA fixed) | 1/40. PubMed: 17116753 |
Target
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FunctionPlays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif.
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Tissue specificityUbiquitously expressed.
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Sequence similaritiesBelongs to the GGA protein family.
Contains 1 GAE domain.
Contains 1 GAT domain.
Contains 1 VHS domain. -
DomainThe VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (AC-LL motif).
The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis.
The unstructured hinge region contains clathrin-binding and an autoinhibitory (AC-LL) motifs.
The GAE domain binds accessory proteins regulating GGAs function. -
Post-translational
modificationsPhosphorylated by CK2 and dephosphorylated by PP2A (By similarity). Phosphorylation of GGA3 allows the internal AC-LL motif to bind the VHS domain and to inhibit the recognition of cargo signals.
Ubiquitinated. -
Cellular localizationGolgi apparatus > trans-Golgi network membrane. Endosome membrane.
- Information by UniProt
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Database links
- Entrez Gene: 23163 Human
- Omim: 606006 Human
- SwissProt: Q9NZ52 Human
- Unigene: 87726 Human
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Alternative names
- ADP ribosylation factor binding protein 3 antibody
- ADP ribosylation factor binding protein GGA 3 antibody
- ADP ribosylation factor binding protein GGA3 antibody
see all
Images
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Western blot - Anti-GGA3 antibody (ab10553)Anti-GGA3 antibody (ab10553) at 1/600 dilution + HEK293 lysate from cells transfected with GFP-GGA3 fusion protein at 35 µg
Secondary
IRDye 800 conjugated Goat-anti-Rabbit IgG [H&L] at 1/10000 dilution
Predicted band size: 78 kDa
A 110kDa band (see arrow) corresponding to the GFP-GGA3 fusion protein is detected by ab10553.
Datasheets and documents
References
This product has been referenced in:
- Koike MA et al. Oligemic hypoperfusion differentially affects tau and amyloid-{beta}. Am J Pathol 177:300-10 (2010). WB ; Mouse . Read more (PubMed: 20472896) »
- Pak Y et al. Transport of LAPTM5 to lysosomes requires association with the ubiquitin ligase Nedd4, but not LAPTM5 ubiquitination. J Cell Biol 175:631-45 (2006). Electron Microscopy ; Human . Read more (PubMed: 17116753) »
