Anti-GGA3 antibody (ab10553)
Key features and details
- Rabbit polyclonal to GGA3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-GGA3 antibody
See all GGA3 primary antibodies -
Description
Rabbit polyclonal to GGA3 -
Host species
Rabbit -
Tested Applications & Species
Application Species WB Human -
Immunogen
Synthetic peptide:
ADPAPNVPPKESAGNS
conjugated to KLH, corresponding to amino acids 400-415 of Human GGA3. -
General notes
Expiration date is 1 year from date of opening.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10553 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
WB |
Human
|
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 78 kDa.
|
Notes |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 78 kDa. |
Target
-
Function
Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the GGA protein family.
Contains 1 GAE domain.
Contains 1 GAT domain.
Contains 1 VHS domain. -
Domain
The VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (AC-LL motif).
The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis.
The unstructured hinge region contains clathrin-binding and an autoinhibitory (AC-LL) motifs.
The GAE domain binds accessory proteins regulating GGAs function. -
Post-translational
modificationsPhosphorylated by CK2 and dephosphorylated by PP2A (By similarity). Phosphorylation of GGA3 allows the internal AC-LL motif to bind the VHS domain and to inhibit the recognition of cargo signals.
Ubiquitinated. -
Cellular localization
Golgi apparatus > trans-Golgi network membrane. Endosome membrane. - Information by UniProt
-
Database links
- Entrez Gene: 23163 Human
- Omim: 606006 Human
- SwissProt: Q9NZ52 Human
- Unigene: 87726 Human
-
Alternative names
- ADP ribosylation factor binding protein 3 antibody
- ADP ribosylation factor binding protein GGA 3 antibody
- ADP ribosylation factor binding protein GGA3 antibody
see all
Images
-
Anti-GGA3 antibody (ab10553) at 1/600 dilution + HEK293 lysate from cells transfected with GFP-GGA3 fusion protein at 35 µg
Secondary
IRDye 800 conjugated Goat-anti-Rabbit IgG [H&L] at 1/10000 dilution
Predicted band size: 78 kDa
A 110kDa band (see arrow) corresponding to the GFP-GGA3 fusion protein is detected by ab10553.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (3)
ab10553 has been referenced in 3 publications.
- Wei R et al. Antagonistic Glucagon Receptor Antibody Promotes a-Cell Proliferation and Increases ß-Cell Mass in Diabetic Mice. iScience 16:326-339 (2019). PubMed: 31203188
- Koike MA et al. Oligemic hypoperfusion differentially affects tau and amyloid-{beta}. Am J Pathol 177:300-10 (2010). WB ; Mouse . PubMed: 20472896
- Pak Y et al. Transport of LAPTM5 to lysosomes requires association with the ubiquitin ligase Nedd4, but not LAPTM5 ubiquitination. J Cell Biol 175:631-45 (2006). Electron Microscopy ; Human . PubMed: 17116753