Key features and details
- Rabbit polyclonal to GGA3
- Suitable for: WB, IHC-P, ICC/IF, ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GGA3 antibody
See all GGA3 primary antibodies
DescriptionRabbit polyclonal to GGA3
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Macaque monkey, Orangutan
- Human breast tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab117086 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 78 kDa.|
|IHC-P||Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use at an assay dependent concentration.|
|ELISA||1/10000 - 1/50000.|
FunctionPlays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the GGA protein family.
Contains 1 GAE domain.
Contains 1 GAT domain.
Contains 1 VHS domain.
DomainThe VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (AC-LL motif).
The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis.
The unstructured hinge region contains clathrin-binding and an autoinhibitory (AC-LL) motifs.
The GAE domain binds accessory proteins regulating GGAs function.
modificationsPhosphorylated by CK2 and dephosphorylated by PP2A (By similarity). Phosphorylation of GGA3 allows the internal AC-LL motif to bind the VHS domain and to inhibit the recognition of cargo signals.
Cellular localizationGolgi apparatus > trans-Golgi network membrane. Endosome membrane.
- Information by UniProt
- ADP ribosylation factor binding protein 3 antibody
- ADP ribosylation factor binding protein GGA 3 antibody
- ADP ribosylation factor binding protein GGA3 antibody
ab117086 has not yet been referenced specifically in any publications.