Overview

  • Product name

    Anti-GGA3 antibody [EPR14340]
    See all GGA3 primary antibodies
  • Description

    Rabbit monoclonal [EPR14340] to GGA3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human GGA3 aa 400-600. The exact sequence is proprietary.
    Database link: Q9NZ52

  • Positive control

    • SH-SY5Y, 293 and A431 cell lines; Human Infiltrating duct carcinoma of breast.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR14340
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab180951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 78 kDa.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif.
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Belongs to the GGA protein family.
    Contains 1 GAE domain.
    Contains 1 GAT domain.
    Contains 1 VHS domain.
  • Domain

    The VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (AC-LL motif).
    The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis.
    The unstructured hinge region contains clathrin-binding and an autoinhibitory (AC-LL) motifs.
    The GAE domain binds accessory proteins regulating GGAs function.
  • Post-translational
    modifications

    Phosphorylated by CK2 and dephosphorylated by PP2A (By similarity). Phosphorylation of GGA3 allows the internal AC-LL motif to bind the VHS domain and to inhibit the recognition of cargo signals.
    Ubiquitinated.
  • Cellular localization

    Golgi apparatus > trans-Golgi network membrane. Endosome membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ADP ribosylation factor binding protein 3 antibody
    • ADP ribosylation factor binding protein GGA 3 antibody
    • ADP ribosylation factor binding protein GGA3 antibody
    • ADP-ribosylation factor-binding protein GGA3 antibody
    • ARF binding protein GGA 3 antibody
    • ARF binding protein GGA3 antibody
    • ARF-binding protein 3 antibody
    • gamma ear-containing antibody
    • GGA 3 antibody
    • GGA3 antibody
    • GGA3_HUMAN antibody
    • Golgi associated gamma adaptin ear containing ARF binding protein 3 antibody
    • Golgi localized gamma ear containing ARF binding protein 3 antibody
    • Golgi-localized antibody
    • KIAA0154 antibody
    see all

Images

  • All lanes : Anti-GGA3 antibody [EPR14340] (ab180951) at 1/5000 dilution

    Lane 1 : SH-SY5Y cell line with NFDM/TBST
    Lane 2 : 293 cell line with NFDM/TBST
    Lane 3 : A431 cell line with NFDM/TBST

    Lysates/proteins at 20 µg per lane.

    Blocking peptides at 5 % per lane.

    Secondary
    All lanes : Peroxidase conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 78 kDa

  • Immunohistochemical analysis of paraffin-embedded Human Iifiltrating duct carcinoma of breast tissue labeling GGA3 using ab180951 at a 1/50 dilution, and counterstained with Hematoxylin. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Flow cytometric analysis of 2% paraformaldehyde fixed SH-SY5Y cells labeling GGA3 using ab180951 at a 1/20 dilutioin, or Rabbit monoclonal IgG (isotype control). Goat anti rabbit IgG (FITC) at a 1/50 dilution was used as a secondary antibody.

References

ab180951 has not yet been referenced specifically in any publications.

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