Product nameAnti-GGA3 antibody [EPR14340]
See all GGA3 primary antibodies
DescriptionRabbit monoclonal [EPR14340] to GGA3
Tested applicationsSuitable for: IHC-P, WB, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human GGA3 aa 400-600. The exact sequence is proprietary.
Database link: Q9NZ52
- SH-SY5Y, 293 and A431 cell lines; Human Infiltrating duct carcinoma of breast.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab180951 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 78 kDa.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionPlays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the GGA protein family.
Contains 1 GAE domain.
Contains 1 GAT domain.
Contains 1 VHS domain.
DomainThe VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (AC-LL motif).
The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis.
The unstructured hinge region contains clathrin-binding and an autoinhibitory (AC-LL) motifs.
The GAE domain binds accessory proteins regulating GGAs function.
modificationsPhosphorylated by CK2 and dephosphorylated by PP2A (By similarity). Phosphorylation of GGA3 allows the internal AC-LL motif to bind the VHS domain and to inhibit the recognition of cargo signals.
Cellular localizationGolgi apparatus > trans-Golgi network membrane. Endosome membrane.
- Information by UniProt
- ADP ribosylation factor binding protein 3 antibody
- ADP ribosylation factor binding protein GGA 3 antibody
- ADP ribosylation factor binding protein GGA3 antibody
All lanes : Anti-GGA3 antibody [EPR14340] (ab180951) at 1/5000 dilution
Lane 1 : SH-SY5Y cell line with NFDM/TBST
Lane 2 : 293 cell line with NFDM/TBST
Lane 3 : A431 cell line with NFDM/TBST
Lysates/proteins at 20 µg per lane.
Blocking peptides at 5 % per lane.
All lanes : Peroxidase conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution
Predicted band size: 78 kDa
Immunohistochemical analysis of paraffin-embedded Human Iifiltrating duct carcinoma of breast tissue labeling GGA3 using ab180951 at a 1/50 dilution, and counterstained with Hematoxylin. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Flow cytometric analysis of 2% paraformaldehyde fixed SH-SY5Y cells labeling GGA3 using ab180951 at a 1/20 dilutioin, or Rabbit monoclonal IgG (isotype control). Goat anti rabbit IgG (FITC) at a 1/50 dilution was used as a secondary antibody.
ab180951 has not yet been referenced specifically in any publications.