RecombinantRabMAb

Recombinant Anti-GGA3 antibody [EPR14340] - BSA and Azide free (ab250282)

Submit a review Submit a question
Western blot - Anti-GGA3 antibody [EPR14340] - BSA and Azide free (ab250282)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGA3 antibody [EPR14340] - BSA and Azide free (ab250282)
  • Flow Cytometry - Anti-GGA3 antibody [EPR14340] - BSA and Azide free (ab250282)
  • Anti-GGA3 antibody [EPR14340] - BSA and Azide free (ab250282)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14340] to GGA3 - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-GGA3 antibody [EPR14340] - BSA and Azide free
    See all GGA3 primary antibodies

  • Description

    Rabbit monoclonal [EPR14340] to GGA3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human GGA3 aa 400-600. The exact sequence is proprietary.
    Database link: Q9NZ52

  • General notes

    Ab250282 is the carrier-free version of ab180951. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab250282 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Affinity purified

  • Clonality

    Monoclonal

  • Clone number

    EPR14340

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab250282 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 78 kDa.

Target

  • Function

    Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif.

  • Tissue specificity

    Ubiquitously expressed.

  • Sequence similarities

    Belongs to the GGA protein family.
    Contains 1 GAE domain.
    Contains 1 GAT domain.
    Contains 1 VHS domain.

  • Domain

    The VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (AC-LL motif).
    The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis.
    The unstructured hinge region contains clathrin-binding and an autoinhibitory (AC-LL) motifs.
    The GAE domain binds accessory proteins regulating GGAs function.

  • Post-translational
    modifications

    Phosphorylated by CK2 and dephosphorylated by PP2A (By similarity). Phosphorylation of GGA3 allows the internal AC-LL motif to bind the VHS domain and to inhibit the recognition of cargo signals.
    Ubiquitinated.

  • Cellular localization

    Golgi apparatus > trans-Golgi network membrane. Endosome membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • ADP ribosylation factor binding protein 3 antibody
    • ADP ribosylation factor binding protein GGA 3 antibody
    • ADP ribosylation factor binding protein GGA3 antibody
    • ADP-ribosylation factor-binding protein GGA3 antibody
    • ARF binding protein GGA 3 antibody
    • ARF binding protein GGA3 antibody
    • ARF-binding protein 3 antibody
    • gamma ear-containing antibody
    • GGA 3 antibody
    • GGA3 antibody
    • GGA3_HUMAN antibody
    • Golgi associated gamma adaptin ear containing ARF binding protein 3 antibody
    • Golgi localized gamma ear containing ARF binding protein 3 antibody
    • Golgi-localized antibody
    • KIAA0154 antibody
    see all

Images

  • Western blot - Anti-GGA3 antibody [EPR14340] - BSA and Azide free (ab250282)
    Western blot - Anti-GGA3 antibody [EPR14340] - BSA and Azide free (ab250282)
    All lanes : Anti-GGA3 antibody [EPR14340] (ab180951) at 1/5000 dilution

    Lane 1 : SH-SY5Y cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : A431 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 78 kDa



    This data was developed using ab180951, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGA3 antibody [EPR14340] - BSA and Azide free (ab250282)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGA3 antibody [EPR14340] - BSA and Azide free (ab250282)
    This data was developed using ab180951, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human Iifiltrating duct carcinoma of breast tissue labeling GGA3 using ab180951 at a 1/50 dilution, and counterstained with Hematoxylin. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Flow Cytometry - Anti-GGA3 antibody [EPR14340] - BSA and Azide free (ab250282)
    Flow Cytometry - Anti-GGA3 antibody [EPR14340] - BSA and Azide free (ab250282)
    This data was developed using ab180951, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde fixed SH-SY5Y cells labeling GGA3 using ab180951 at a 1/20 dilutioin, or Rabbit monoclonal IgG (isotype control). Goat anti rabbit IgG (FITC) at a 1/50 dilution was used as a secondary antibody.
  • Anti-GGA3 antibody [EPR14340] - BSA and Azide free (ab250282)
    Anti-GGA3 antibody [EPR14340] - BSA and Azide free (ab250282)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab250282? Please let us know so that we can cite the reference in this datasheet.

ab250282 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab250282.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com