RecombinantRabMAb

Recombinant Anti-GGCT antibody [EPR17107] (ab198503)

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Western blot - Anti-GGCT antibody [EPR17107] (ab198503)
  • Immunocytochemistry/ Immunofluorescence - Anti-GGCT antibody [EPR17107] (ab198503)
  • Immunocytochemistry/ Immunofluorescence - Anti-GGCT antibody [EPR17107] (ab198503)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGCT antibody [EPR17107] (ab198503)
  • Anti-GGCT antibody [EPR17107] (ab198503)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17107] to GGCT
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-GGCT antibody [EPR17107]
    See all GGCT primary antibodies

  • Description

    Rabbit monoclonal [EPR17107] to GGCT

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human GGCT aa 1-100. The exact sequence is proprietary.
    Database link: O75223

  • Positive control

    • WB: HepG2, 293 and HeLa whole cell lysates; Human fetal kidney and fetal brain lysates. IHC-P: Human kidney tissue. ICC/IF: HeLa and MCF7 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab198503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
IHC-P 1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).

Target

  • Function

    Catalyzes the formation of 5-oxoproline from gamma-glutamyl dipeptides and may play a significant role in glutathione homeostasis. Induces release of cytochrome c from mitochondria with resultant induction of apoptosis.

  • Sequence similarities

    Belongs to the gamma-glutamylcyclotransferase family.
  • Information by UniProt

  • Database links

    • Alternative names

      • C7orf24 antibody
      • CRF21 antibody
      • Cytochrome c-releasing factor 21 antibody
      • Gamma glutamyl cyclotransferase antibody
      • Gamma-glutamylcyclotransferase antibody
      • GCTG antibody
      • GGC antibody
      • Ggct antibody
      • GGCT_HUMAN antibody
      see all

    Images

    • Western blot - Anti-GGCT antibody [EPR17107] (ab198503)
      Western blot - Anti-GGCT antibody [EPR17107] (ab198503)
      All lanes : Anti-GGCT antibody [EPR17107] (ab198503) at 1/1000 dilution

      Lane 1 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
      Lane 2 : 293 (Human embryonic kidney) whole cell lysate
      Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
      Lane 4 : Human fetal kidney lysate
      Lane 5 : Human fetal brain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 21 kDa
      Observed band size: 21 kDa


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunocytochemistry/ Immunofluorescence - Anti-GGCT antibody [EPR17107] (ab198503)
      Immunocytochemistry/ Immunofluorescence - Anti-GGCT antibody [EPR17107] (ab198503)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling GGCT with ab198503 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on MCF7 cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

      The negative controls are as follows:-
      -ve control 1: ab198503 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
      -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

       

    • Immunocytochemistry/ Immunofluorescence - Anti-GGCT antibody [EPR17107] (ab198503)
      Immunocytochemistry/ Immunofluorescence - Anti-GGCT antibody [EPR17107] (ab198503)

      Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling GGCT with ab198503 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on HeLa cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

      The negative controls are as follows:-
      -ve control 1: ab198503 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
      -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGCT antibody [EPR17107] (ab198503)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGCT antibody [EPR17107] (ab198503)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling GGCT with ab198503 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm and weak nuclear staining on Human kidney tissue is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Anti-GGCT antibody [EPR17107] (ab198503)
      Anti-GGCT antibody [EPR17107] (ab198503)

    References (1)

    Publishing research using ab198503? Please let us know so that we can cite the reference in this datasheet.

    ab198503 has been referenced in 1 publication.

    • He Z  et al. Ras Downstream Effector GGCT Alleviates Oncogenic Stress. iScience 19:256-266 (2019). PubMed: 31400748

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