Recombinant
RabMAb

Recombinant Anti-GGCT antibody [EPR17107] (ab198503)

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Overview

  • Product name

    Anti-GGCT antibody [EPR17107]
    See all GGCT primary antibodies

  • Description

    Rabbit monoclonal [EPR17107] to GGCT

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human GGCT aa 1-100. The exact sequence is proprietary.
    Database link: O75223

  • Positive control

    • WB: HepG2, 293 and HeLa whole cell lysates; Human fetal kidney and fetal brain lysates. IHC-P: Human kidney tissue. ICC/IF: HeLa and MCF7 cells.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab198503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
IHC-P 1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).

Target

  • Function

    Catalyzes the formation of 5-oxoproline from gamma-glutamyl dipeptides and may play a significant role in glutathione homeostasis. Induces release of cytochrome c from mitochondria with resultant induction of apoptosis.

  • Sequence similarities

    Belongs to the gamma-glutamylcyclotransferase family.
  • Information by UniProt

  • Database links

    • Alternative names

      • C7orf24 antibody
      • CRF21 antibody
      • Cytochrome c-releasing factor 21 antibody
      • Gamma glutamyl cyclotransferase antibody
      • Gamma-glutamylcyclotransferase antibody
      • GCTG antibody
      • GGC antibody
      • Ggct antibody
      • GGCT_HUMAN antibody
      see all

    Images

    • Western blot - Anti-GGCT antibody [EPR17107] (ab198503)
      Western blot - Anti-GGCT antibody [EPR17107] (ab198503)
      All lanes : Anti-GGCT antibody [EPR17107] (ab198503) at 1/1000 dilution

      Lane 1 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
      Lane 2 : 293 (Human embryonic kidney) whole cell lysate
      Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
      Lane 4 : Human fetal kidney lysate
      Lane 5 : Human fetal brain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 21 kDa
      Observed band size: 21 kDa


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunocytochemistry/ Immunofluorescence - Anti-GGCT antibody [EPR17107] (ab198503)
      Immunocytochemistry/ Immunofluorescence - Anti-GGCT antibody [EPR17107] (ab198503)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling GGCT with ab198503 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on MCF7 cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

      The negative controls are as follows:-
      -ve control 1: ab198503 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
      -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

       

    • Immunocytochemistry/ Immunofluorescence - Anti-GGCT antibody [EPR17107] (ab198503)
      Immunocytochemistry/ Immunofluorescence - Anti-GGCT antibody [EPR17107] (ab198503)

      Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling GGCT with ab198503 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on HeLa cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

      The negative controls are as follows:-
      -ve control 1: ab198503 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
      -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGCT antibody [EPR17107] (ab198503)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGCT antibody [EPR17107] (ab198503)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling GGCT with ab198503 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm and weak nuclear staining on Human kidney tissue is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    References

    ab198503 has not yet been referenced specifically in any publications.

    Publishing research using ab198503? Please let us know so that we can cite the reference in this datasheet.

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