Key features and details
- Rabbit polyclonal to GGCX
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GGCX antibody
See all GGCX primary antibodies
DescriptionRabbit polyclonal to GGCX
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Fusion protein corresponding to Human GGCX. (Near the C terminal; NP_000812. The identity of the protein fusion partner is GST).
Database link: P38435
- MCF7, HeLa and 293T cell lysates; Human liver cancer tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 49% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab197982 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 88 kDa.|
|IHC-P||1/50 - 1/200.|
FunctionMediates the vitamin K-dependent carboxylation of glutamate residues to calcium-binding gamma-carboxyglutamate (Gla) residues with the concomitant conversion of the reduced hydroquinone form of vitamin K to vitamin K epoxide.
Involvement in diseaseDefects in GGCX are a cause of combined deficiency of vitamin K-dependent clotting factors type 1 (VKCFD1) [MIM:277450]; also known as multiple coagulation factor deficiency III (MCFD3). VKCFD leads to a bleeding tendency that is usually reversed by oral administration of vitamin K.
Defects in GGCX are the cause of pseudoxanthoma elasticum-like disorder with multiple coagulation factor deficiency (PXEL-MCFD) [MIM:610842]. This syndrome is characterized by hyperlaxity of the skin involving the entire body. Important phenotypic differences with classical PXE include much more severe skin laxity with spreading toward the trunk and limbs with thick, leathery skin folds rather than confinement to flexural areas, and no decrease in visual acuity. Moreover, detailed electron microscopic analyzes revealed that alterations of elastic fibers as well as their mineralization are slightly different from those in classic PXE.
Sequence similaritiesBelongs to the vitamin K-dependent gamma-carboxylase family.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- FLJ26629 antibody
- Gamma glutamyl carboxylase antibody
- Gamma-glutamyl carboxylase antibody
All lanes : Anti-GGCX antibody (ab197982) at 1/400 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 88 kDa
Exposure time: 40 seconds
Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling GGCX with ab197982 at 1/50 dilution.
ab197982 has been referenced in 1 publication.
- Bordoloi J et al. Gamma-glutamyl carboxylated Gas6 mediates the beneficial effect of vitamin K on lowering hyperlipidemia via regulating the AMPK/SREBP1/PPARa signaling cascade of lipid metabolism. J Nutr Biochem 70:174-184 (2019). PubMed: 31226525