Key features and details
- Goat polyclonal to GGCX - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GGCX antibody - C-terminal
See all GGCX primary antibodies
DescriptionGoat polyclonal to GGCX - C-terminal
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Monkey, Gorilla, Common marmoset, Orangutan
- Human kidney tissue; Human muscle lysate.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab219757 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 88 kDa).|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionMediates the vitamin K-dependent carboxylation of glutamate residues to calcium-binding gamma-carboxyglutamate (Gla) residues with the concomitant conversion of the reduced hydroquinone form of vitamin K to vitamin K epoxide.
Involvement in diseaseDefects in GGCX are a cause of combined deficiency of vitamin K-dependent clotting factors type 1 (VKCFD1) [MIM:277450]; also known as multiple coagulation factor deficiency III (MCFD3). VKCFD leads to a bleeding tendency that is usually reversed by oral administration of vitamin K.
Defects in GGCX are the cause of pseudoxanthoma elasticum-like disorder with multiple coagulation factor deficiency (PXEL-MCFD) [MIM:610842]. This syndrome is characterized by hyperlaxity of the skin involving the entire body. Important phenotypic differences with classical PXE include much more severe skin laxity with spreading toward the trunk and limbs with thick, leathery skin folds rather than confinement to flexural areas, and no decrease in visual acuity. Moreover, detailed electron microscopic analyzes revealed that alterations of elastic fibers as well as their mineralization are slightly different from those in classic PXE.
Sequence similaritiesBelongs to the vitamin K-dependent gamma-carboxylase family.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- FLJ26629 antibody
- Gamma glutamyl carboxylase antibody
- Gamma-glutamyl carboxylase antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human kidney tissue labeling GGCX with ab219757 at 5 µg/ml.
Anti-GGCX antibody - C-terminal (ab219757) at 0.3 µg/ml + Human muscle lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 88 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab219757 has not yet been referenced specifically in any publications.