Overview

  • Product name

    Anti-GGH antibody [EPR8014]
    See all GGH primary antibodies
  • Description

    Rabbit monoclonal [EPR8014] to GGH
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human GGH aa 250-350. The exact sequence is proprietary.

  • Positive control

    • MCF7, HepG2 and HeLa cell lysates
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as Glutamyl hydrolase gamma

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab138495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      Hydrolyzes the polyglutamate sidechains of pteroylpolyglutamates. Progressively removes gamma-glutamyl residues from pteroylpoly-gamma-glutamate to yield pteroyl-alpha-glutamate (folic acid) and free glutamate. May play an important role in the bioavailability of dietary pteroylpolyglutamates and in the metabolism of pteroylpolyglutamates and antifolates.
    • Sequence similarities

      Belongs to the peptidase C26 family.
      Contains 1 gamma-glutamyl hydrolase domain.
    • Cellular localization

      Secreted > extracellular space. Lysosome. Melanosome. While its intracellular location is primarily the lysosome, most of the enzyme activity is secreted. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links

    • Alternative names

      • Conjugase antibody
      • folylpolygammaglutamyl hydrolase antibody
      • gamma glutamyl hydrolase antibody
      • Gamma-Glu-X carboxypeptidase antibody
      • Gamma-glutamyl hydrolase (conjugase, folylpolygammaglutamyl hydrolase) antibody
      • Gamma-glutamyl hydrolase antibody
      • GGH antibody
      • GGH_HUMAN antibody
      • gH antibody
      see all

    Images

    • All lanes : Anti-GGH antibody [EPR8014] (ab138495) at 1/1000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 36 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab138495 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab138495.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up