Product nameAnti-GGH antibody [EPR8014]
See all GGH primary antibodies
DescriptionRabbit monoclonal [EPR8014] to GGH
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide within Human GGH aa 250-350. The exact sequence is proprietary.
- MCF7, HepG2 and HeLa cell lysates
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as Glutamyl hydrolase gamma
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20ºC.
Dissociation constant (KD)KD = 3.78 x 10 -11 M Learn more about KD
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab138495 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 36 kDa.|
FunctionHydrolyzes the polyglutamate sidechains of pteroylpolyglutamates. Progressively removes gamma-glutamyl residues from pteroylpoly-gamma-glutamate to yield pteroyl-alpha-glutamate (folic acid) and free glutamate. May play an important role in the bioavailability of dietary pteroylpolyglutamates and in the metabolism of pteroylpolyglutamates and antifolates.
Sequence similaritiesBelongs to the peptidase C26 family.
Contains 1 gamma-glutamyl hydrolase domain.
Cellular localizationSecreted > extracellular space. Lysosome. Melanosome. While its intracellular location is primarily the lysosome, most of the enzyme activity is secreted. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- Conjugase antibody
- folylpolygammaglutamyl hydrolase antibody
- gamma glutamyl hydrolase antibody
All lanes : Anti-GGH antibody [EPR8014] (ab138495) at 1/1000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 36 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab138495 has not yet been referenced specifically in any publications.