Anti-Ghrelin antibody (ab129383)
Key features and details
- Rabbit polyclonal to Ghrelin
- Suitable for: IHC-P, WB, ELISA
- Reacts with: Mouse
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Ghrelin antibody
See all Ghrelin primary antibodies -
Description
Rabbit polyclonal to Ghrelin -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ELISAmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Cat, Dog, Human, Rhesus monkey -
Immunogen
Synthetic peptide corresponding to Mouse Ghrelin aa 1-100 conjugated to keyhole limpet haemocyanin.
Database link: Q9EQX0-1 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129383 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 13 kDa.
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ELISA |
Use at an assay dependent concentration.
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Notes |
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IHC-P
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 13 kDa. |
ELISA
Use at an assay dependent concentration. |
Target
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Function
Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation.
Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility. -
Tissue specificity
Highest level in stomach. All forms are found in serum as well. Other tissues compensate for the loss of ghrelin synthesis in the stomach following gastrectomy. -
Sequence similarities
Belongs to the motilin family. -
Post-translational
modificationsO-octanoylation or O-decanoylation is essential for ghrelin activity. The O-decanoylated forms Ghrelin-27-C10 and Ghrelin-28-C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26. A small fraction of ghrelin, ghrelin-28-C10:1, may be modified with a singly unsaturated carboxylic acid.
Amidation of Leu-98 is essential for obestatin activity. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 51738 Human
- Entrez Gene: 58991 Mouse
- Entrez Gene: 59301 Rat
- Omim: 605353 Human
- SwissProt: Q9UBU3 Human
- SwissProt: Q9EQX0 Mouse
- SwissProt: Q9QYH7 Rat
- Unigene: 590080 Human
see all -
Alternative names
- Appetite regulating hormone antibody
- Ghrelin 27 antibody
- Ghrelin 28 antibody
see all
Datasheets and documents
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Datasheet download
References (11)
ab129383 has been referenced in 11 publications.
- Duan J et al. Comparison of the effects of different calorie amounts of enteral nutrition in hypercatabolism associated with ghrelin-POMC in endotoxemic rats. Nutr Metab (Lond) 19:28 (2022). PubMed: 35428321
- Cui XB et al. ADAR1 deficiency protects against high-fat diet-induced obesity and insulin resistance in mice. Am J Physiol Endocrinol Metab 320:E131-E138 (2021). PubMed: 33252250
- Sagsöz H et al. The expressions of some metabolic hormones (leptin, ghrelin and obestatin) in the tissues of sheep tongue. Anat Histol Embryol 49:112-120 (2020). PubMed: 31568599
- Chen H et al. Ghrelin suppresses migration of macrophages via inhibition of ROCK2 under chronic intermittent hypoxia. J Int Med Res 48:300060520926065 (2020). PubMed: 32485129
- Nunez-Salces M et al. Nutrient-sensing components of the mouse stomach and the gastric ghrelin cell. Neurogastroenterol Motil 32:e13944 (2020). PubMed: 32666613