Overview

  • Product name

  • Description

    Rabbit polyclonal to Ghrelin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Cat, Dog, Human, Rhesus monkey
  • Immunogen

    Synthetic peptide corresponding to Mouse Ghrelin aa 31-49 conjugated to keyhole limpet haemocyanin.
    Sequence:

    Glu - His - Gln - Lys - Ala - Gln - Gln - Arg - Lys - Glu - Ser - Lys - Lys - Pro - Pro - Ala - Lys - Leu - Gln -KLH


    Database link: Q9EQX0

  • Positive control

    • This antibody gave a positive result in IHC in the following FFPE tissue: Mouse Stomach.

Properties

Applications

Our Abpromise guarantee covers the use of ab129383 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250. Predicted molecular weight: 13 kDa.
ELISA 1/256000.
IHC-P Use a concentration of 0.2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation.
    Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility.
  • Tissue specificity

    Highest level in stomach. All forms are found in serum as well. Other tissues compensate for the loss of ghrelin synthesis in the stomach following gastrectomy.
  • Sequence similarities

    Belongs to the motilin family.
  • Post-translational
    modifications

    O-octanoylation or O-decanoylation is essential for ghrelin activity. The O-decanoylated forms Ghrelin-27-C10 and Ghrelin-28-C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26. A small fraction of ghrelin, ghrelin-28-C10:1, may be modified with a singly unsaturated carboxylic acid.
    Amidation of Leu-98 is essential for obestatin activity.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Appetite regulating hormone antibody
    • Ghrelin 27 antibody
    • Ghrelin 28 antibody
    • Ghrelin and obestatin prepropeptide antibody
    • Ghrelin antibody
    • Ghrelin/obestatin prepropeptide antibody
    • ghrl antibody
    • GHRL_HUMAN antibody
    • Growth hormone releasing peptide antibody
    • Growth hormone secretagogue antibody
    • Growth hormone-releasing peptide antibody
    • M46 protein antibody
    • Motilin related peptide antibody
    • Motilin-related peptide antibody
    • MTLRP antibody
    • Obestatin antibody
    • Protein M46 antibody
    see all

Images

  • IHC image of Ghrelin staining in Mouse Stomach formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab129383, 0.2 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:

  • Sun X  et al. Incretin dysfunction and hyperglycemia in cystic fibrosis: Role of acyl-ghrelin. J Cyst Fibros N/A:N/A (2019). Read more (PubMed: 30738804) »
  • Qu R  et al. Ghrelin protects against osteoarthritis through interplay with Akt and NF-?B signaling pathways. FASEB J 32:1044-1058 (2018). Read more (PubMed: 29070587) »
See all 2 Publications for this product

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