Anti-Ghrelin antibody (ab217011)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Ghrelin antibody
See all Ghrelin primary antibodies -
Description
Rabbit polyclonal to Ghrelin -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse, Human
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Immunogen
Synthetic peptide within Mouse Ghrelin aa 24-51 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Sequence:GSSFLSPEHQKAQQRKESKKPPAKLQPR
Database link: Q9EQX0 -
Positive control
- Rat stomach tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab217011 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/500. |
Target
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Function
Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation.
Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility. -
Tissue specificity
Highest level in stomach. All forms are found in serum as well. Other tissues compensate for the loss of ghrelin synthesis in the stomach following gastrectomy. -
Sequence similarities
Belongs to the motilin family. -
Post-translational
modificationsO-octanoylation or O-decanoylation is essential for ghrelin activity. The O-decanoylated forms Ghrelin-27-C10 and Ghrelin-28-C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26. A small fraction of ghrelin, ghrelin-28-C10:1, may be modified with a singly unsaturated carboxylic acid.
Amidation of Leu-98 is essential for obestatin activity. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 51738 Human
- Entrez Gene: 58991 Mouse
- Entrez Gene: 59301 Rat
- Omim: 605353 Human
- SwissProt: Q9UBU3 Human
- SwissProt: Q9EQX0 Mouse
- SwissProt: Q9QYH7 Rat
- Unigene: 590080 Human
see all -
Alternative names
- Appetite regulating hormone antibody
- Ghrelin 27 antibody
- Ghrelin 28 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody (ab217011)Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat stomach tissue labeling Ghrelin with ab217011 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
Protocols
Datasheets and documents
References
ab217011 has not yet been referenced specifically in any publications.
