Our Abpromise guarantee covers the use of ab64325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/5000. Predicted molecular weight: 13 kDa.Can be blocked with Ghrelin peptide (ab199421).


  • Function

    Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation.
    Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility.
  • Tissue specificity

    Highest level in stomach. All forms are found in serum as well. Other tissues compensate for the loss of ghrelin synthesis in the stomach following gastrectomy.
  • Sequence similarities

    Belongs to the motilin family.
  • Post-translational

    O-octanoylation or O-decanoylation is essential for ghrelin activity. The O-decanoylated forms Ghrelin-27-C10 and Ghrelin-28-C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26. A small fraction of ghrelin, ghrelin-28-C10:1, may be modified with a singly unsaturated carboxylic acid.
    Amidation of Leu-98 is essential for obestatin activity.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • Appetite regulating hormone antibody
    • Ghrelin 27 antibody
    • Ghrelin 28 antibody
    • Ghrelin and obestatin prepropeptide antibody
    • Ghrelin antibody
    • Ghrelin/obestatin prepropeptide antibody
    • ghrl antibody
    • GHRL_HUMAN antibody
    • Growth hormone releasing peptide antibody
    • Growth hormone secretagogue antibody
    • Growth hormone-releasing peptide antibody
    • M46 protein antibody
    • Motilin related peptide antibody
    • Motilin-related peptide antibody
    • MTLRP antibody
    • Obestatin antibody
    • Protein M46 antibody
    see all


This product has been referenced in:

  • Aghajanova L  et al. Diminished endometrial expression of ghrelin and ghrelin receptor contributes to infertility. Reprod Sci 17:823-32 (2010). Read more (PubMed: 20616368) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Baboon Tissue sections (brain hypothalamus)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.01M Citrate buffer pH6.0
brain hypothalamus
Blocking step
(agent) for 10 minute(s) · Concentration: 10% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Aug 28 2018


Thank you for your enquiry.

The antibody is not capable of detecting obestatin, as it is raised against a peptide derived from mature ghrelin. It appears in a search using the search term "obestatin" because obestatin is a product of the GHRL gene, as is ghrelin. The antibody ab41704, on the other hand, is in fact raised against rat obestatin.

I understand that this creates confusion, but our search function often includes all known products of a given gene, in this case the GHRL gene. The processing of the precursor is described at the UniProt database entry for human GHRL, accession Q9UBU3. Here is the URL:


This search strategy is meant to be useful but in this instance, I think it is too comprehensive. I hope my explanation makes sense. If you have any further questions or concerns, please contact me.

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Thank you for contacting us.

The antibody is generated against a peptide derived from the mature form. This sequence of amino acids is contained also in the precursor forms (prepro and pro), so in theory the antibody should be capable of recognizing those forms, though they may be present so transiently that the amounts are below the detection limit of the antibody.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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